4 li THE BOTANICAL MAGAZINE. 



[Vol. XXI. No. 21;?. 



The solutions used as outside media are as follows : 



7n> mol. solution of cane sugar + '/iooo mol. H.,S0 4 

 : 7io mol. solution of cane sugar 

 7a mol. solution of dextrose + Viooo mol. H 2 S0 4 

 7« niol. solution of dextrose 

 7a mol. solution of levulose + 7iooo mol. H L ,SO, 

 7a mol. solution of levulose 

 7io mol. solution of KNO 

 7 mol. solution of Asparagin 

 The capillary solutions tried are: 1) 



7so mol. solution of sodium malate 

 7oo mol. solution of sodium maleinate 

 V»o mol. solution of calcium maleinate 

 V.jo mol. solution of ammonium fumar'ate 

 V.-.o mol. solution of ammonium succinate 

 7so mol. solution of patassium citrate 

 7so mol. solution of ammonium asparaginase 

 "7i„ mol. solution of dextrose 

 2 /io mol. solution of levulose 

 7k. mol. solution of cane sugar 

 7m mol. solution of chinine hydro-chloride 

 7io mol. solution of ephedrin hydro-chloride 

 Concentrated solution of egg-albumin 

 Archegonial contents of CycasP 

 We tried the various combinations of the above mentioned 

 solutions, and in not one case have been able to observe either 

 positive or negative chemotactic reaction. It is especially to 

 be noticed that the spermatozoids have shown no reaction 

 towards the archegonial contents. On the other hand we have 

 observed that some Pteridophyta-spermatozoids are attracted 

 l)\ the archegonial contents of Cycas. The following experi- 

 ments were made with the spermatozoids of Equisetum: 



o Into the capillary solutions we added usually cane sugar or other substances 

 whir h were used for outside media, in about the same concentration as the latter, to 

 avoid the difference of osmotic pressure of two solutions. 



-> Thick, mucilaginous fluid of the egg as well as more clear watery iluid of the 

 Lis, 



