ill 



TBE BOTANICAL MAGAZINE. 



[Vol. XXI No. 248. 



KC1(0.1) 



All cells healthy after 10 weeks. 



K < . 0.3 



All cells healthy for 3 weeks, then a gradual change 

 of the chloroplast-spiral took place, it contracted, 

 moved to the cell ends and formed an longer starch. 

 Gradually also the nucleus suffered. 1 ) 



KNO S (0.15) 



All cells normal and rich in starch after 15 days; 

 later on gradual death ; some cells alive after 42 days 



K s SO 4 (0.3j 



A number of cells still perfectly healthy after 50 

 days. Injury commenced after 28 days. Parasites 

 numerous, like in the former ca«e. 



CaCL(0.2) 



All cells alive after 80 days. In a few cells the 

 nucleus has moved to the wall. Much starch, no 

 parasites. Gradual death afterwards. 



Ca(XO 3 ),(0.2) 



All cells healthy after 50 days, no parasites. 



Ca(SOJ(0.2) 



All cells healthy after 80 days, no parasites. Later 

 on the filaments became yellowish. Much starch. 

 Later on gradual death. 



KCl(0.15)+MgCl 2 (0.1) 



Most cells killed after 10 days; the still living show 

 chlorophyll body attacked, its lobes being retracted 

 and sometimes the spiral torn into fragments. But 

 such injured cells were still alive 4 weeks later. 



D Buch injured cells had still the normal turgor, but the nucleus and very probably 

 also the chlorophyll body were killed. The nucleus had contracted to an irregularshaped 

 ma-- and was lying on the side. Such cases were observed years ago by one of us when 

 highly diluted solutions of oxalic acid acted on the cells. These cells with the 

 cytoplasm alive and the nucleus killed recalled Gkiiaksimows Spirogyra cells without 

 any nucleus obtained by the influence of low lemperature or anaesthetics after the cell 

 division had made :i -tart. The substance contained in the chlorophyll body may serve 

 tain tin- lit*'- of the cytoplasm in ease the; assimilation of carbon in the former 

 bad ceaa d. Bach cells without nucleus are capable to live for six weeks (Ueuassimow). 



