1 -20 THE BO T. 1 Nil ,1 /. M, I OAZINR L Yo i. XX i. No. 245 



and before the mitotic division only a few globules are left 

 scattering among the large amount of the achromatic sub- 

 stance. 



B. Just before the decrease of chromatic substance the 

 nuclear membrane thickens apparently, owing to the deposition 

 of numerous chromatic granules in its inner surface. Similar 

 ease may be found in Synchytrium decipiens and Stevens assumed 

 it in the latter fungus to be the first step of the transformation 

 oi the membrane into a granular halo around the karyokinetic 

 figures. In S. Puerarise no connection between the membrane 

 and the halo is ascertained, for the former becomes somewhat 

 faint and begins to disappear after the decrease of chromatic 

 substance. 



9. The fate of the ground substance of the primary nucleolus 

 is nearly similar to that of the secondary- nucleoli. It produces 

 pseud opodia-like processes and disintegrates into the radiating 

 striatums, often carrying the chromatic globules. At this time 

 the nuclear membrane disappears entirely and the remaining 

 chromatic globules are finally transformed into chromosomes. 



10. The spindle is then formed at the center of the achro- 

 matic striations. Its origin is not apparent. Sometimes a 

 remnant of the nucleolus may be seen near the spindle. The 

 striations become gradually inconspicuous and change into the 

 granular mass surrounding the spindle. It corresponds exactly 

 to what Stevens denoted as a halo which originates, according 

 to him, from the nuclear membrane. At later stage the halo 

 becomes faint and gradualh' disappears. 



11. The chromosomes .are globular or slightly oblong in 

 form and five- in number. 



12. 'fhe daughter chromosomes fuse together at the telo- 

 phase and form a round mass at the pole. This mass represents 

 the nucleolus of the daughter nucleus (a, b). It shows that the 

 chromatic substanee is contained in the nucleolus and the 

 chromosomes originate from the latter. 



L3« At the resting stage the structure of the daughter 

 nucleus and the nuclei of the succeeding division is quite the 

 Bame as that of the primary nucleus. The process of the 



