March, 1917.1 NAGAI.—THE ACTIOS OF OXIDASE (XV ANTHOOYANIN. 



73 



cyanin extract which is of intense red colour, is just as easily 

 decolourized as a weakly acidified one. Neither do certain 

 mineral salts, such as calcium chloride, magnesium sulphate, 

 alum, and others any retardation even in saturation. In the 

 case of the plant juice, however, the reaction is interrupted or 

 largelv retarded by weak acids and neutral mineral salts added. 

 The case of calcium chloride may be mentioned. The colour of 

 anthocvanin extract is altered by an addition of calcium salts.' 

 With the anthocvanin from the chrysanthemum flower, the 

 colour changes from reddish blue to green, while with that 

 from Perilla leaves, to reddish violet. The action of the pressed 

 juice of At -miliaria edodes is retaJed considerably even by the 

 presence of a small amount of calcium salt. Observe the 

 following: 



Table VI. 



cone, of CaCl 2 {%) 



cc of en- 

 zyme BOl. 



ce antho- 

 cvanin. 



intensity of colour- after 

 40 in. 110 m. 



2.0 



1.25 



.<; 

 o 

 2.5 (control) 



deep green 

 deep green 

 green (44) 

 colourless (0) 



green 



green 

 pale green (28) 

 colourless (0) 



deep green (100) deep green (100) 



It has been shown so far that the decolourization is Auc to 

 the decomposition of anthocvanin molecule by oxidising agents, 

 and if that be the case the reaction can be accelerated l>y 

 certain catalizers. In fact, it i^ observed that the addition "I :i 

 9m all amount of pulverised animal charcoal promotes the rate 

 ol decolourization in a marked degree, especially in a dilute 

 solution. 



1 Cf. Shibata, K. Sim: \ ia. Y. and Ka-iuu m.i. I.: The J 

 ■I:i| in N i. III Nov. 1016. 

 - I i. hi n tin blucki i indie tl i idii 



Pharmai eut. 



