ABSENCE OF GUM AND PRESENCE OF A NEW DIGLUCOSIDE. 31 



due evidently to the small amount of free tannin which 

 they contain, because the " Stringybark " kinos all give 

 dense precipitates with these. The glucoside gave similar 

 results in every other respect with the original " Ironbark" 

 kino. The kinos of the " Stringybarks," etc., and the 

 "Ironbarks," as well as the glucoside, gave precipitates 

 and behaved similarly with 5, 6, 7, 8, and 10, although with 

 5 the precipitate with the glucosidal kinos was longer in 

 forming. With 9 the "Ironbark" kinos gave a pinkish 

 colour and a pink-brown precipitate, the " Stringybark " 

 kinos giving a purplish colour and a purple-brown precipi- 

 tate. With 10 at first a greenish precipitate, and on adding 

 ammonia in excess a dense dark brown flocculent precipitate 

 was obtained. 



Preparation of the glucoside. — About half a pound of 

 the freshly exuded kino of E. paniculata was dissolved in 

 the smallest possible quantity of water, it was strained 

 through cloth and to the clear liquid about 3 litres of alcohol 

 added and allowed to stand over night. The precipitate 

 had then separated as a solid somewhat dark coloured cake. 

 The alcohol was removed, the cake broken up, washed in 

 fresh alcohol, dissolved in the smallest quantity of water 

 and again precipitated by alcohol. This process was 

 repeated for the third time, and although the alcohol was 

 scarcely coloured, yet the precipitate was somewhat dark 

 and this was difficult to remove even by boiling with 

 animal charcoal for a long time. The precipitate was 

 finally dried at a low temperature and powdered. When 

 heated at 100° 0. it darkened considerably. The powder 

 as thus prepared was of a cinnamon colour, had scarcely 

 any odour, was astringent to the taste, was readily soluble 

 in cold water, but quite insoluble in alcohol and in ether. 

 Attempts to remove it from solution by solvents were not 

 successful. It gave reactions similar to those of the 



