ABSENCE OF GUM AND PRESENCE OF A NEW DIGLUCOSIDE. 33 



when the temperature was raised to 28° 0. or 30° C. When 

 prepared for the polarimeter the sugar was found to be 

 without rotation. 



Preparation of the osazone. — The osazone was prepared 

 by heating on the water bath with phenylhydrazine in 

 dilute acetic acid, but owing to its marked solubility in 

 acetic acid it was difficult to prepare in quantity. The 

 osazone was a yellow crystalline powder, soluble in hot 

 water but separated again in microscopic crystals on cool- 

 ing. It was somewhat soluble in alcohol and ether-alcohol, 

 but did not dissolve readily in either ether, benzene or 

 chloroform. It melted at 176-178° C. 



Decomposition of the ''''kino red" by caustic potash, — 

 A portion of the " kino red " obtained from the kino of E. 

 paniculata was fused with 10 times its weight of caustic 

 potash, adding a little water, and heating for half an hour 

 at 150 — 170° C. The melt, which was of a very dark brown 

 colour, was dissolved in water, acidified with sulphuric acid 

 and extracted by ether. No volatile acid was detected 

 when the solution was acidified. On evaporating the ether 

 a crystalline substance was obtained ; this was dissolved 

 in water, acetate of lead added in excess, and the precipi- 

 tate removed. The filtrate was acidified with sulphuric 

 acid and extracted by ether, but only the merest trace of 

 a substance was obtained and this did not give the reaction 

 for phloroglucinol. The lead precipitate was decomposed 

 by sulphuric acid, filtered, and extracted by ether. The 

 ether on evaporation gave a well marked crystallised mass 

 which when purified from water melted at 195 — 197° C, 

 and gave all the reactions for protocatechuic acid, which 

 acid it evidently was. 



The "kino red" obtained in a similar manner from the 

 kino of E. pilularis, and from two or three other species 



C— June 1 1904. 



