July. 192 VJ 



BACTERIAL GALL ON MILLETIA PLAST. 



Ill 



Inoculation Experiments 



Exp. I. June 21, 1918. 



Organism ; cultured on glueose agar plate for 10 days by room 

 temperature. 



Plants ; 2 years old white flower species in pot. The bark of the 

 plants was wet as it rained from the morning. 



Methods ; the organism was inoculated by needle puncture on the 

 two-years-old stem and covered with bell jars. The in- 

 oculated plants were kept on the floor of our laboratory. 



Results ; as following table. 



Wo. of 



plants 



Inoculations 

 made 



July 21 



Aug. 23 



at 4 inoculations tumor j the dimension of the largest 

 growth occur. knot is 3x2x1,5 mm. 



9 pricks with- 

 out infection 



no tumor growth 



no tumor growth 



Exp. II. Aug. 28, 1918. 



Organism ; cultured on glucose agar for 24 hours by 32 ~C. 



Plants ; some fruit trees and leguminous plants. About 2 hours 



before inoculation sterilized water was sprayed, and once 



more just before to wet the bark. 

 Methods ; as Exp. I. 

 Results ; as following table. 



Inoculated 

 plants 



Inoculations 

 made 



Aug. 13 



Sep. 1 



Milletia Horibunda 



6 



4 galls produced 



.alls produced 



Maackia amurensis 



6 



no galls produced 



no galls produced 



Glediischia horrida 



6 



do. 



do. 



Lespedeza sp. 



6 



do. 



do. 



Persimmon 



6 



do. 



do. 



Pear 



6 



do. 



do. 



Apple 



6 



do. 



do. 



From the above experiments we were induced to believe that 

 the organism must have a causal relation to the gall formation and 

 considering it to be a new species we propose to call the organism 

 Bacillus milletiae. Observations on the morphological and cultural 

 characters of this organism are given in the following lines. 



