112 THE BOTANICAL MAGAZINE. CV°i. xxxiv. No. m. 



Morphological Characters of the Causal Organism 



The organism is a motile rod with rounded ends, measuring 

 0,9-2,5 X 0,4-0, 6/J., the prevailing dimension is 2,1 X 0,6//. 



On glucose agar it occurs in solitary and enveloped with capsule. 

 No spores have been observed even in 6 month' culture. It is motile 

 by means of 7-8 peripheral flagella. It stains easily with basic anilin 

 dyes, but not by Gram's stain. Involution forms (partly thickened 

 filamentous bodies) are formed in acid peptone water. 



Cultural and biochemical Characters 



(All cultural experiments were done by 32°C, except those with 

 gelatine media) Agar poured plate. On the surface of neutral (litmus) 

 peptonized beef agar, the colonies are about 1-1,5 mm. in diameter after 

 2 days. They are round, flat, smooth, waxy yellow, shining, opaque 

 and entire margin with granular structure ( X 60). After 4-5 days 

 they thin as if dry down. Burried colonies are lenticular and those 

 which were between the glass and agar are round, white and with 

 coarse granular center. 



Agar slant. In 24 hours yellow, opaque, glistening, smooth, flat, 

 filiform streak is produced along the inoculated line. After 48 hours 

 the colour changes to greenish yellow, and after 3 days a metallic 

 lustered membrane covers the bacterial mass. 



Glucose agar slant. The growth is moderate, filiform, raised, 

 smooth, glistening and opaque. Within 12 hours it is white in colour, 

 later yellow to brownish yellow, and some putrefactive odour is 

 produced. The condensed water cloud heavily. 



Agar stab. The growth along the inoculated puncture is uniform 

 and no characteristic signs. 



Glucose agar stab. The growth is as that of agar stab, but the 

 browning of the media occurs from the surface after 10 days. 



Gelatine poured plate (20-23°C). Within 3 days a visible growth 

 occurs. After 7 days the surface colonies raised as hemisphere of 

 brownish yellow colour. 



Gelatine stab (18-20°C). The stab growth is filiform. After 8 

 days liquefaction began and it progresses in funnel shape. 



Potato cylinder. After 28 hours it grows filiform on steamed 

 potato cylinder spreading at the bottom. The streak is flat, shining, 

 smooth and light yellow. Later it becomes brown and covered with 

 metallic lustered membrane. 



Beef bouillon. Strong clouding occurs within 24 hours. After 3-7 

 days narrow ring of light yellow and heavy precipitates are formed. 



