July, 1920.] 



BACTERIAL GALL ON MILLETIA PLANT. 



113 



Milk. Inoculated milk slowly clears from the surface without 

 coagulation. 



Litmus milk. Litmus blues from the top downward on the 3rd 

 day. After 7 weeks the blue colour bleaches out. 



Litmus whey. Litmus turns on 2nd day to red, on 4th day to 

 light red and on 6th day to light blue. The bleaching occurs at the 

 bottom on 4th day. 



Peptone water. Weak clouding after 48 hours. The reaction of 

 the medium becomes decided alkali. 



Nitrate bouillon. Strong clouding and ring formation occurs, the 

 nitrate is reduced to nitrite. 



Conjac. Growth is feeble, and the streak is filiform, thin, glisten- 

 ing and brownish yellow. No liquefaction is observed. 



Saccharose potato agar. The growth is abundant, filiform, flat, 

 glistening, semi-transparent and the bacterial mass falls to the bottom, 

 owing to its overgrowth. The colour after 24 hours is white, 48 

 hours later yellow. 



Aerobism. This organism grows better in aerobic condition. On 

 glucose agar plate in hydrogen gas or oxygen removed air, it produces 

 somewhat smaller, thinner and fainter coloured colonics than in air. 



Fermentation tube. The tests of fermentation were made by \% 

 Witte Peptone water, added \% carboncompounds to be tested ; namely 

 saccharose, lactose, maltose, laevulose, galactose, mannit, and glycerin, 

 litmus being used as indicator. The clouding was equally on both 

 closed and open arm, but no gas was formed on airy cultures up to 7 

 weeks. Acid production was recognized on every culture out of 

 glycerine tube, and the strongest is saccharose tube and the weakest 

 laevulose tube. The heaviest clouding occurs in saccharose and maltose 

 tube, and the weakest in glycerine tube. 



Toleration of acids. The growth of this organism in neutral 

 (phenolphthalein) saccharose peptone water added 0,5 0,3 0,2 0,1 and 

 0,05 per cent, respectively, of citric; tartaric, oxalic and acetic acid is 

 as follows : 



\. Acid 



Citric 



, Tartaric 



Oxalic 



Acetic 





Note?. 



% XT 



acid 



acid 



acid 



acid 





0.3 



__ 





_ 



_ 



— 



indicates no growth. 



0.2 



+ 



+ 



± 



- 



+ 



,, good growth. 



0.1 



+ 



* 



4f 



+ 



4f 



„ better growth. 



0.05 



+ 



# 



-H- 



# 



% 



„ best growth. 



0.00 



-ff 



-H- 



4r 



^ 







