July, 1920.] BACTERIAL GALL ON MILLET IA PLANT. H5 



Enzyme Excretion. Two weeks' culture of the peptone water of 

 B. milletiae added thymol as 0,1°/o, was used for enzyme studying. 



Starch paste of 0,5^ with 0,1% thymol was added to the liquid 

 above mentioned and kept 37 C C. about 24 hrs. But no reducing sugar 

 was produced and shows negative result of diastase bilding. 



Also \°/o saccharose water gave a negative results in searching 

 invertase excretion. 



Peroxydase is detected by steeping the bacterial mass in hydrogen 

 peroxyd solution. 



Literature cited 



Smith, E. F. ar.d Towxsexd. Bull. 213, Bureau of Plant Industry, U.S. Dep. 



of Agric. 1911. 

 Smith, E. F. Centralblatt fur B^kt. 11. AB. Bd. 20,p. 89, 1907. 

 Ceaytox O. Smith. A Bacterial Disease of Oleander, Bacillus Oleae Arcang) 



Teev. Bot. Gaz., Vol. 42, p. 301, 1906. 

 Pa yarino, L., Kiv. d. Bat. Veg. 5: 65, 1911. 



Explanation of Plate II 



1. Galls formed on the brar.ch of Milletia floriiunda var. Brachybotrys MATSM. by 



artificial inoculation, after six weeks. 



2. Gallformation on the naturally infected branch of M'dletia floribunda Matsm. 



3. Causal organism, stained by the method of Loeffler. (4x1/12 Leiz) 



4. Causal organism with cipsnle. 



