Preliminary Notes on the Development of the 

 Carpospores of Porphyra tenara Kjellm. 



By 



Kintaro Okanmra, Keisuke Onda and Michitaro Higashi. 



(Studied on the Scholarship of the Department of Education. 



(With Plate III.) 



Prof. Yendo published an interesting observation which lias at* 

 tracted the attention, not only of algologists but biologists at latere. 

 The paper of Prof. Yendo refers to the development of carpospores of 

 Porphyra leucosticta Thur. stating that the supposed male and female 

 swarming gametes are produced from germinated carpospores. 1 ' This 

 interesting conclusion by Prof. Yendo has been the motive for a revised 

 study of the subject which we have prosecuted. 



Last winter we collected several specimens of Porphyra tencra 

 Kjellm. at different times from material cultured in Tokyo Bay. The 

 specimens were grown in deep Peter's dishes (7-9 cm. in diameter, 

 and 5 cm. high.) containing natural sea water with or without 

 artificial nutritive compounds, and covered with glass covers. The 

 natural sea water which we employed as culture medium was taken 

 one mile off from Tatenoshima in Tate\-ama Bay, which is situated some 

 44 miles from Tokyo. As the sea water is rich in salinity, having a 

 specific gravity of 1.025, we diluted it by mixing water from the Tokyo 

 aqueduct until the specific gravity was 1.020. This diluted sea water 

 was carefully filtered and 0.2 per cent Sodium Nitrate and a trace of 

 Calcium Phosphate were added as nutrients. 



For the sake of convenience we shall refer to the cultures without 

 artificial nutritive compounds as hunger cultures and to the others as 

 nutrition cultures. 



1) Yejstdo K. : The Germination and Development of Some Marine Algae (T.B. 

 M. Vol. XXXIII. Bfo. 388, 1919). 



