88 J. B. CLELAND AND T. H. JOHNSTON. 



were not seen in any of these forms. The pigment was 

 usually at or near the ends of the parasite. The other 

 forms, with protoplasm much more definitely stained, are 

 probably mostly female gametocytes, though some are 

 perhaps schizonts. The pigment was perhaps more massive 

 in these. Frequently a definite vacuole was present at 

 one end. 



A number of gametocytes, both male and female, in the 

 stage following on this were noticed. In both forms the 

 parasite, after reaching the above stage seemed to contract 

 towards its centre, becoming firstly a flattened oval and 

 finally spherical in a position lateral to the host nucleus. 

 Having reached this last form, it appeared as a distinct 

 bulging in a distorted red corpuscle, which, from the large 

 number of ruptured blood cells encountered, would then 

 appear to be more fragile than normal, probably from the 

 tenseness of the bulging. Forms quite free from the host 

 cell were not infrequent. The two sexual types could be 

 quite easily followed in this evolution. In the male type, 

 in the elongated oval form, was disclosed in favourable 

 specimens an elongated pinkish nucleus. In the mature 

 male gametocyte, the nucleus was frequently observed as 

 an irregularly round body in a protoplasm apparently quite 

 unstained. The pigment appeared as scattered fine granules 

 sometimes as a circle round the nucleus. In one example, 

 the male gametocyte was very much larger than others 

 seen, its diameter being equal to that of the long diameter 

 of a red cell; its nucleus was divided into three irregular 

 masses and one rod shaped mass, separated by considerable 

 intervals; its pigment was scattered as fine granules. In 

 another of normal size, several delicate flagella-like bodies 

 were noticed attached to its circumference (? possibly 

 microgametes). 



The mature female forms were all of one size, were well 

 stained, granular; a vacuole was often seen; and the pig- 



