1885.] Microscopy. 329 
“ Before hardening the brains were inflated with Miiller’s fluid, 
so as to preserve the natural proportion of the cavities. After 
treatment with alcohol, they were placed for a week in dilute 
carmine. Calberla’s egg-mass was employed as before, except 
that the ventricles were injected with the mass before hardening. 
The delicate parts of the brain-roof were thus retained. It ap- 
pears now that celloidin may be used- for this purpose to equal, if 
not to greater advantage in results, and with considerable econ- 
omy of time. The sections were cut in absolute alcohol, were 
then floated upon a slide in consecutive order, from twenty to fifty 
at a time, and were covered with a delicate slip of blotting paper 
during treatment with oil of cloves.” 
Imbedding—1. The egg-mass was prepared by shaking the 
white and yolk of egg together, with three drops of glycerine to 
each egg, and then well filtered through coarse cloth. 
2. The bath is then prepared as follows: 
a, Outside is a large water pan for boiling with the Bunsen 
burner, &c. 
Cover sf. th 
ice pulled. 
zar pet- N- 
eka 
--Large water pan 
05% Alcohot -~~ wade 
per cent alcohol. : 
c. Within the glass dish is placed a piece of coarse wire netting 
which supports the imbedding box, raising it above the alcohol. 
` 3. The box, made of paper in the usual way, and one-fourth 
filled with the imbedding mass, is kept in the bath until the mass 
is hardened enough to support the brain. The brain is next 
placed on the hardened stratum and covered with the fresh mass. 
The second stratum is hardened i an enoni to hold the brain in 
lace, and then a third is added, filling the box. 
‘i 4. The whole mass must now be allowed to harden through 
and through, iring about fifteen minutes. 
5. The Sandenag completed by passing the box through 
three grades of alcohol—eighty, ninety and a hundred per cent, 
allowing it to remain twenty-four hours in each. . 
