332 General Notes. [ March, 
tion from both sides. It consists of four pieces of glass of the 
shape and size seen in the figure (a 6c d), and a cover-glass, g, 
which serves as the object-bearer. The two glasses, æ and 4, are 
painted on one side with chloroform balsam, and then connected 
turned over and the middle portions of the glass bars, c and d, 
painted with balsam; and a thin glass cover, g, placed so as to 
rest on the pointed sides of cand d. This glass (g) bears the 
object in dammar, which is covered by another very thin glass. 
The object, lying between two thin cover-glasses, can be viewed 
from both sides with the highest powers. 
THE PREPARATION OF MeRostastic Ova.—A. Reftilian Ova} 
—1. The ova taken from the oviduct are opened in a dilute solu- 
tion of osmic acid (one per cent) and then the white removed as 
far as possible. 
2. The osmic acid is then turned off and a weak solution of 
chromic acid (% per cent) added; twenty-four hours. 
3- With a sharp, fine pair of scissors cut around the germinal 
area, just outside its margin; and after it has been completely 
encircled with the incision, float it carefully off from the body of 
e yolk, 
4. The yolk and acid are next removed, and a copious supply 
of clean water added, which must be several times renewed. 
5. Calberla’s fluid (glycerine, water and absolute alcohol in 
equal parts) three hours. 
ardened in ninety per cent alcohol. 
7. Stained in Bohm’s carmine acetate twenty-four hours. 
B. Teleostean Ova (T. fario).—1. Chromic acid (% per cent) 
twenty-four hours. 
2. Distilled water two hours. The.egg-membrane expands, 
and may now be easily removed, 
3- Washed in distilled water twelve hours. - 
4. Absolute alcohol, glycerine and aq. dest. in equal parts 
four hours. 
5. Absolute alcohol. 
_ 6. Bohm’s carmine acetate one to two days. 
_7- Mixture of water (fifty vols.), glycerine (fifty vols) and mu- 
riatic acid (a half vol.), for a few minutes. . 
_ 8. Washed in water, four to five hours. 
9. Absolute alcohol twelve hours, preparatory to imbedding in 
paraffine. 
t 
__ Bouw’s CARMINE ACETATE, —1. Carmine (four grms.) pulverized 
_ 4m 200 grms. water, 
__. 2, Ammonia added by drops until the solution becomes cherry- 
_ ted (the carmine should now be fully dissolved). 
os + Kupffer. His and Braune. Archiv. Anat. Abth., 1882, p, 4. 
