438 Some New Infusoria. [ May, 
body. When forcibly extruded through the influence of the 
glycerole of tannin, the distal end of each, for about one-tenth of 
the entire length, is conspicuously thickened, so that the tricho- 
cyst seems to be supplemented by the addition of a minute 
pyramid (Fig. 5). Occasionally, before the animalcule’s death, 
when suffering from the application of a very dilute solution of 
the glycerole, it then gradually assuming an evenly ovoid form and 
becoming pale and ghostly, and always after the extrusion of the 
trichocysts and their removal from the body, the cuticular eleva- 
tions are replaced by equally minute, regularly disposed, parallel- 
ogrammic depressions, as if the escaping trichocysts had left 
empty spaces which were filled by the sinking of the cortex. 
The nucleus and nucleolus are not always constant either in rela- 
tion to each other or to a special part of the body. The former 
is sometimes, and normally it would seem, subcentrally located, 
yet sometimes being near the dorsum, again nearest the ventral 
surface, and still again in the anterior extremity, being seldom 
seen behind the body center. The laterally attached nucleolus is 
almost as uncertain in its relative connection with the nucleus, 
becoming at times entirely detached. 
Conjugation has been observed, union taking place between 
the latero-ventral surfaces. Reproduction is by transverse fission, 
the nucleus previously becoming much elongated, the dividing 
plane passing through its center. In some instances, soon after 
the beginning of genetic union, the nucleus assumes a finely 
striated appearance, gradually growing more and more indistinct 
in contour until it finally becomes indistinguishable from the sur- 
rounding endoplasm. 
Paramecium trichium, sp. nov.—Body soft and flexible, ovate, somewhat com- 
pressed, three times as long as Praa, piee and TES inflated ` posteriorly, both 
extremities rounded, the ventral st hat flattened; adoral fossa extending 
to the center of the ventral aspect from the left obliquely toward the right, deepest 
and widest anteriorly, this part of the body apparently folded obliquely toward the 
left-hand side; oral aperture followed by a distinct, tubular, ciliated pharyngeal 
peeenges trichocysts abundant, arranged vertically and apparently elevating the 
spherical projections roughening the entire body and giving it in optical section a 
cr ted outline, their distal extremities, when forcibly extruded, conspicuously and 
pyramidally thickened ; nucleus ovate, usually subcentrally placed, with a laterally 
attached nucleolus; contractile vesicle double, spherical, anteriorly located ; anal 
aperture ventro-terminal, Length of body ;4,, of trichocysts qg inch. Habitat: 
3 Santen mass of fungoid and bacterial growth on the surface of an infusion 
