328 General Notes. [August, 
MICROSCOPY.! 
Some HısrorocicaL Meruops sy DR. C. S. Minot.—Miiler's 
Fluid—In hardening in Müller’s fluid it is important to keep the 
jar with specimens and fluid in a cold place, best near freezing, 
for three or four days. The delicate tissues are then much bet- 
ter preserved; probably the same is true of Erlicki’s fluid. Using 
Müller’s fluid at a high temperature is bad for epithelia. - 
Beales Carmine— 
Carmine. I gram. 
Ainoa ie OR ers oe RT 3 c.c. 
Pure glycerine. sors 96 čt, 
Distilled water i 96 c.é: 
Aoh G per centu Iana a AA pee rer lvlees 24 A 
into the original bottle. à 
rom the carmine solution the sections are placed in water and 
washed thoroughly, after which they are placed for 1-3 minutes 
in hydrochloric acid diluted with water until it tastes about like 
sharp vinegar. They are finally again washed in water and are 
then ready to mount in the usual manner. 
Employed in this way Beale’s carmine is one of the most val- 
uable of histological staining fluids, both for general use and also 
more especially for the central nervous system. If by any chance 
. 
the sections are overstained, the superfluous color may be ex- 
dilute one part of the stock solution with twenty parts alcohol. 
The alcoholic solution is far more convenient than the aqueous. 
Imbedding in Celloidin—The object after having been tho- 
= roughly dehydrated in alcohol is placed for twenty-four hours n 
= a@mixture of equal parts of strong alcohol and pure ether. If 
_ this mixture is kept long a little ether must be added from time 
1 Edited by Dr. C. O. WHITMAN, Mus. Comp. Zool., Cambridge, Mass. 
