1304 General Notes. [December, 
blood enclosed between two surfaces, one of which is concave; 
the latter may be obtained either by using a curved cover glass or 
by making a depression in the slide. Norris recommends a 5 p. c. 
solution of sulphate of soda as an excellent preservative for the 
extremely delicate “invisible corpuscles.” The special interest 
of Bizzozero’s work lies in the relation supposed by him to exist 
between the colorless corpuscles and the coagulation of the 
blood. This author believes the fibrin to be derived from the 
disintegration of the colorless disks, and the following are the 
principal arguments introduced by him in support of this view: 
1. Liquids which have a tendency to prevent coagulation pre- 
serve also more or less completely the blood plates from destruc- 
tion; atnong these liquids are solutions of sodium sulphate, 
magnesium sulphate, sodium nitrate, strong sodium bicarbonate, 
dilute sodium carbonate, glycerine, and 0.75 per cent sodium 
chloride to which some methyl violet has been added. — 
‘2. Experiments made upon blood kept within the uninjured 
blood-vessel, after the manner of Briicke, showed that as long 
as the blood remained uncoagulated, the blood plates kept their 
shape, while the rapid coagulation of shed blood was always pre- 
ceded by a destruction of the plates and the formation of granu- 
lar masses from them. : ae 
3. When a drop or two of blood was whipped with slen 
threads for about 50-55 seconds, the threads then withdrawn an 
slightly washed with 0.75 per cent sodium chloride meee 
methyl violet, and then examined under the microscope Il)" 
same liquid, they were found covered with a layer of plates ad 
gether with some white corpuscles. If the whipping Was” 
tinued longer the layer of plates became a granular mass OF i 
transformed into a film of fibrin. He was able to heidi is 
tent to watch this process, the deposition of the tesa 
fusion into a granular mass and the subsequent formant ae 
‘rin, by observing under the microscope a thread placed ye 
current of blood, thus reversing the process of whipping. Jastin 
4. When to a liquid containing fibrinogen and pE : 
only, some of the colorless blood plates adhering to a 7 smn 
sence of a forig? 
he coagulation 
ble 
nificant or wanting altogether. The clot was not pei ae 
cocytes adhering to the thread, for when bits 0 l of bone, 
bodies such as the spleen, lymph glands, ulation Tè 
etc., were added to the “proplastic ” liquid, no coag ime 
sulted, except in the case of the last substance, W ‘til the white 
caused a slight coagulum. The conclusion is, that ia leucocytes 
blood corpuscles are shown to be different from 
