31 



Remarks. — This species which is also common in Europe and North America, 

 was described as Uredo Agrimoniae {DC.) by Schrceter, (1) its teleuto- 

 spore stage being unknown for a long time. 



In 1890 Dietel (2) found the teleutospores of this species on the speci- 

 mens of Agrimonia pilosa Collected in Siberia and also on those of Agri- 

 monia Eupatoria Collected in Iowa (North America). According to him, 

 the teleutospores are formed in the epidermal cells on the under surface 

 of the leaf: that is, they are intracellular. He considered it to belong 

 to the genus Thecopsora and applied the name of Thecopsora Agri- 

 monies (DC.) Dietel. 



In our country also, the uredo stage of this fungus is commonly met 

 with wherever the host plant is found. Fortunately, I have found its 

 teleutospores, during my study, in the spring of 1896, in the old speci- 

 mens collected by Prof. Miyabe and myself near Sapporo ; and also 

 in those collected later in the vicinity of Hirosaki. 



In our specimens, the teleutospores are formed under the epidermal 

 layer on the under surface of the leaf, and never in the ceil cavities as 

 was stated by Dietel. The mode of the formation of the teleutospores 

 according to my observation is as follows : — 



The hyphfe traversing between the mesophyll-cells of the leaf, make a 

 vigorous growth between the epidermis and the underlying parenchyma 

 cells. The tips of the hyphal branches gradually swell up (Fig. 2), 

 and develop into the unicellular mother teleutospores, which by succes- 

 sive cell divisions by longitudinal septa, many smaller daughter spores 

 are formed. (Fig. 3, 4.). Their size and shape vary according to the 

 condition of nutrition and the space allowed for them. (Fig. 5, 6.). 



Other morphological characters of the teleutospores agree well with 

 those described by Dietel. From the place of the formation of the teleuto- 

 spores, it is evident, that the fungus under consideration must be 



placed under the genus Pucciniastrum. In regard to the uredo-stage 

 of the fungus, our specimens agree well with the descriptions by both 

 Schroeter and Dietel. As Dietel especially remarked in his paper 

 already alluded to, the uredospores are formed singly upon the basidia 

 and never in chains as in the Caeoma.* 



1) Die Pilze Schlesiens B d. I, p. 374. 



2) P. Dietel, *" Beschreibimg der Teleutosporenform von Uredo Agrirnocise (DC)," 

 Hedwigia Bd. 29,1890, p. 152. 



* I was informed by Prof. Miyabe, wben my note was ready for press, that Dietel (in 

 "UredineEe brasilienses a el. E. Ule lectae" in Hedwigia Bd. XXXVI, 1897. p. 33) enumerated 

 the present species under the name of Pucciniastrum Agrimonies, (DC.) without any remarks. 



