23 



which is attached to the anterior part of the body, is stained light red, 

 as it is of the cytoplasmic origin. 13 



As far as I know, the sperrnatozoid of Pellia is the largest one 

 hitherto known among the Hepatic^, 2, and as the sperrnatozoid of Makinoa 

 is much larger than that, so it may be the largest among the Hepaticae. 

 Thus Makinoa crispata is a very good object for the study of the struc- 

 ture and development of plant sperrnatozoid, which I am hoping to under- 

 take in the coming year. 



The diagnosis of our new liverwort is given as. follows : — 



Makinoa crispata, nov. gen. et nov. sp. 



Plant thalloid, dichotomously branched ; dioecious, bearing the sexual 

 organs on the dorsal median surface, near the apex of the thallus ; the 

 ventral surface with numerous rhizoids on the median portion : archegonia 

 grouped, on the apex of the dorsal surface, covered by a slightly dentated 

 involucre : antheridia in group, immersed in a depression on the apex 

 of the thallus, bordered by a crescent-shaped ridge on the hinder part ; 

 sperrnatozoid of very large size: capsule of the sporogonium oblong elliptical, 

 dark-brown in color, with long seta ; elater long, tapering at both ends, 

 with 2 spirals fused into a continuous mass of thickened wall in one 

 side ; spore globular, greenish ; calyptra cylindrical projected much above the 

 involucre. 



Fruiting in April, the sexual organs being ripen in the same month. 



The plant grows in the valley of Mt. Kiyosumi, in the province of 

 Awa, on the Pacific coast, collected by Mr. MakiDO. It- was also found 

 before by Mr. Faurie at Akita, in the northern part of Japan and sent 

 to Mr. Stephani. It was after this specimen that he first described the 

 present plant under the name Pellia crispata. 



In closing I wish to express my hearty thanks to Mr. Stephani for 

 his kind suggestion. 



Botanical Institute, College of Science, 

 Imperial University, Tokyo. 



Nov. 15th, 1898. 



1) For the further discussion of the structure and development of the sperrnatozoid of 

 plant, see ! Belajeff, Ueber Bau und Entwickelung der Spermatozoiden der Pflanzen, Flora, 

 1894. 



2) Campbell, The Structure and Development of the Mosses and Ferns, 1895, P. 92. 



