94 b. brXdley. 



6. On arabinose, acid and gas is produced by all but 

 the hog cholera type, generally under four days, but certain 

 of the food poisoning group took longer — over a week to 

 do that. The hog cholera cultures do not attack arab- 

 inose under twenty-one days. 



7. Salicin is rendered slightly acid by three cultures; by 

 the rest it is unaffected up to three weeks. 



8. The Gaertner group produce on litmus a transient and 

 often very feeble acidity, followed later by a reversal of 

 the process generally shown by marked alkalinity. The 

 subdivision into A and B types is one of degree, and cannot 

 be strictly maintained bio-chemically as linking types are 

 found between the extremes, but may be of interest in 

 tracing the relationship of various strains of Gaertner type, 

 pseudo-Gaertner and other colon bacilli. 



9. Ordinary milk is after a month perceptibly cleared by 

 the vast majority of Gaertner type strains. There is a 

 close relationship between tlie degree of alkali formation 

 and the "clearing." 



10. The indol reaction tested on the seventh day in 

 peptone water by the sulphuric acid and nitrite method is 

 variable, but never more than slight. This test is unreliable. 

 No indol can be demonstrated by the benzaldehyde method 

 under identical conditions. 



11. Morphology, motility, growth on gelatin agar or 

 potato are of no assistance whatever in the grouping of 

 these organisms. 



12. Fourteen stock cultures isolated at the Bureau from 

 local sources and tested in the same way as the European 

 series, give results generally speaking confirmatory of the 

 above, except that one culture from a food poisoning 

 epidemic slowly affected saccharose. Salicin Was attacked 

 slowly by this, and by another culture from a food poison- 



