292 DR THOMAS H. BRYCE ON 
The two dyes were applied successively, and not in a mixture, according to a method 
described by Dr GuLLanpD, and communicated to me by Dr Goopatu. The sections 
were stained first for about five minutes with a saturated watery solution of eosin, and 
then after washing, with a saturated watery solution of methylene blue for two or 
three minutes. They were then washed and differentiated if necessary in 90° alcohol, 
dehydrated and cleared in pure xylol. 
The sections stained by this method are as bright after nine months as they were 
at first. 
Il. SrrucTuRE OF THE ERYTHROCYTES. 
(a) Resting Corpuscles. 
The red blood corpuscles are oval biconvex discs, varying in size from 42 to 50» in 
leneth, 30 to 36 in breadth, and 12 to 15» in thickness. ‘The nucleus occupies the 
centre of the dise (Pl. I. fig. 1, Pl. IV. fig. 32). It is also oval in shape, measuring 
20 to 27 » in length, 12 to 15 in breadth, and 9 to 12 » in thickness. . 
(1) Cytoplasm. 
The corpuscle is surrounded by a delicate membrane. The cell body shows a 
peripheral ring or band, within which there is a coarse meshwork structure. The 
meshwork is not very regular, but the thickness of the sections intensifies the 
appearance of irregularity. The meshes are from 3 to 4 in diameter. The whole | 
reticulum centres on the nucleus, having a general radial direction from nucleus to | 
periphery. At the nodal points there are strongly refractile granules of considerable 
size. 
In some corpuscles the fibrille of the reticulum in the central nuclear portion of the 
corpuscle are arranged as parallel running threads between the nucleus and the periphery, | 
but it is not quite clear how far this is a normal appearance. 
The staining reactions of the meshwork are as follows :—With iron hematoxylin 
it is grey, while the microsomes are black (Pl. I. figs. 1 and 2); with methylene blue 
and eosin, the meshwork stains bright red and the microsomes are dark red spots; 
with triacid it is yellow, and the microsomes stand out as darker yellowish-brown points. 
In some favourable stainings with the last-named mixture the alveoli had a faint pink 
tinge. 
In all the larger corpuscles there is a large vacuole, with structureless contents, 
showing no differential reaction to any of the stains used. 
Round the equator of the cell there is a remarkable band about 3 in diameter. It 
forms a complete peripheral ring, when the corpuscle is seen on the flat (Pl. I. fig. 1, 
Pl. IV. fig. 32). In the fixed cell its appearance is distinctly fibrillar. The fibrillee run 
