THE SOURCE OF THE AMNIOTIC AND ALLANTOIC FLUIDS IN MAMMALS. 77 







Foetus 



Allantoic 



Amniotic 







Weight. 



Amount. 



Amount. 





The two sacs removed at the beginning of the 





experiment contained : — 



1 





109 



24 



12 



2 





105 



28 



15 





The six sacs removed after death contained : — 



3 





103 



23 



16 



4 





102 



36 



19 



5 





103 



31 



15 



6 





98 



31 



13 



7 





91 36 



18 



8 



Foetus left in sac and fixed in formalin. 



Total. 



Per cent. 



none 





0-008 



00036 



0-00008 



0-004 



0-0008 



0-00057 



0-0004 



0-0006 



None of the fluids were fluorescent except those of 7, which were distinctly so. 



The allantoic and amniotic fluids of 4 and 7 gave a slight but distinct reaction of 

 salicylic acid with ferric perchloride. 



The combined urine collected from the bladders was strongly fluorescent, but the 

 urine of 6 was free of fluorescence. The combined urine also gave a strong reaction 

 of salicylic acid with ferric perchloride. 



The foetuses, the allantoic and amniotic fluids, and about 1 c.cm. of the combined 

 urine were sent to Mr Kerr, who reports as follows, as regards the presence of Nal : — 



Presence of Nal. 

 Sample. 



Foetuses (1 and 2 check), 142 grms. 



(3 to 7), 223 grms. 

 Foetal urine, 2 c.c. 

 Allantoic fluid, 140 c.c. 

 Amniotic fluid, 60 c.c. 



Experiment I. — Guinea-pig, 570 grms., nearly full time. Injected 0'29 grm. sodium 

 ferrocyanide in 4|- minutes. Killed after 15 minutes. Urine and kidney of mother 

 gave marked reaction. Foetal fluid and foetal tissues gave no reaction. Maternal 

 placenta gave a green colour. 



Experiment II. — Cat, about 2000 grms., nearly full time. 0*8 grm. sodium 

 ferrocyanide in 13 minutes. Animal killed after 20 minutes. Kidney and urine of 

 mother gave very marked reaction. Fluids, tissues, and urine of foetuses gave no 

 reaction. 



Experiment III. — Rabbit, about 24 days. Sodium sulphindigotate (not fully 

 saturated), 38 c.c. injected in 11 minutes. The maternal urine was blue- 

 First sac removed 9 minutes after injection. 

 Fluids not coloured. 

 Urine ,, 



Kidney 



