78 



D. NOEL PATON, B. P. WATSON, AND J. KERR ON 



Second sac, 19 minutes after injection. 



Fluid faint green. 



Urine not coloured. 



Kidney ,, 

 Third sac, 34 minutes after injection. 



Fluid not coloured. 



Urine ,, 



Kidney „ 



When mother killed, urine in bladder was green, but the kidneys were not coloured. 



Experiment IV. — Rabbit, about 20 days. Fluorescin, - 5 per cent., 10 c.c. in 6 

 minutes. Five minutes after, abdomen opened ; abundant fluorescent ascitic exudation 

 found. Ten minutes after, one cornu of uterus with three sacs excised. Membranes 

 appeared fluorescent, but fluid was not so. The foetal blood was not fluorescent. 

 Fifteen minutes after, the other cornu was removed, and gave the same characters. 

 The urine and tissue of the mother were strongly fluorescent. 



Experiment V. — Guinea-pig, about 650 grms. 20 c.c. of - 5 per cent, fluorescin in 8 

 minutes. Animal killed after 1 5 minutes. All the maternal tissues markedly fluorescent. 

 No colour in fluids or in tissues of foetuses. 



Experiment VI. — Rabbit, almost full time. 20 c.c. 10 per cent, sodium iodide in 

 12 minutes. Animal killed 13 minutes after. Two sacs, one with exomphalotic and 

 anencephalic foetus, other with normal foetus but no fluids. 



Experiment VII. — Rabbit, 12-14 days. One sac excised as control. 20 c.c. sodium 

 iodide (10 per cent.) in 13 minutes. Killed 12 minutes after. Amniotic fluid of six sacs 

 mixed — also allantoic. Report : — 



Sample. 

 Maternal urine 

 Amniotic fluid 

 Allantoic fluid 

 Control foetus . 

 Foetuses (6) after injection 



Presence of Iodide. 

 strongly marked, 

 none. 



distinct trace, 

 none, 

 strongly marked. 



Experiment VIII. — Rabbit, 17 days. Control sac excised. 20 c.c sodium iodide 

 (10 per cent.) in 9 minutes. Animal died 6 minutes after injection stopped. The 

 fluids of the remaining six sacs were mixed. Report : — 



.Sample. 

 Maternal urine 

 Mixed fluids after injection 

 Foetus .... 

 Control fluids . 

 Control foetus . 



Presence of Iodide, 

 very strongly marked, 

 distinct trace, 

 strongly marked, 

 none, 

 none. 



Experiment IX. — Already described in detail. 



Experiment X. — Guinea-pig, nearly full time. One foetus of 92 grms. 20 c.c. of 

 1 per cent, fluorescin in 10 per cent, sodium iodide in 5 minutes. Animal killed 10 



