THE SOURCE OF THE AMNIOTIC AND ALLANTOIC FLUIDS IN, MAMMALS. 



79 



minutes after. Maternal blood fluorescent, but urine not so. Fluids not distinctly 

 fluorescent. Foetal blood distinctly fluorescent. Report : — 



Sample. 



Presence of Iodide.* 



Maternal blood 



. - 0-026 grm. 



Foetus ..... 



none. 



Fluids . . . . . 



none. 



Foetal blood .... 



0-002 grm. 



Maternal urine 



0-0003 grm. 



Experiment XL — Guinea-pig, nearly full time. Two foetuses about 80 grms. 

 1 7 "5 c.c. of same solution in 5 minutes. Animal killed after 10 minutes. Maternal 

 blood fluorescent. Foetal blood fluorescent. Fluids slightly fluorescent. Report : — 



Sample. Presence of Iodide.* 



Maternal blood ........ 0-012 grm. 



Foetuses 0-002 grm. 



Foetal blood . . . . . . . . . present. 



Fluids 0-00006 grm. 



Experiment XII. — Cat. 45 c.c. solution of sodium iodide 10 per cent., sodium 

 salicylate 5 per cent., sodium sulpliindigotate to saturation, were injected in 23 minutes. 

 The animal died. Mucous membranes, all connective tissues, and muscles intensely 

 blue. Medulla of kidney blue, cortex green. Liver and spleen not blue ; but when 

 cut and exposed to air the surface of liver became greenish blue. Intestines deep blue, 

 but when left covered by other coils lost their blue colour. Urine intensely blue, with 

 red shimmer. Wall of uterus deep blue. Blood-plasma deep blue. 



Four sacs. Foetuses almost full time, weighing about 75 grms. each. 



Foetal organs and blood gave no trace of blue. 



Foetal urine, about 1 c.c, gave no trace of blue, but one drop gave a faint brown with 

 FeCl 3 , indicating the presence of salicylic acid. 



Allantoic fluid of brown colour. No blue tinge. Gave faint deepening of brown 

 with FeCl 3 . 



Amniotic fluid. — No trace of blue. Slight brown with FeCl 3 . Report states 

 that there is a considerable quantity of iodide in the maternal urine (17 c.c.) and 

 in the maternal blood (56 c.c), but only a trace in the allantoic fluid (7 c.c.) and in 

 the amniotic fluids (13 c.c), and a trace in the four foetuses (300 grms.) = 0*0012 grm. 



Twelve experiments have thus been performed — five on rabbits, four on guinea-pigs, 

 two on cats, and one on a dog. 



Sodium ferrocyanide has been injected twice, sodium sulphindigotate twice, sodium 

 iodide seven times, fluorescin five times, and sodium salicylate twice. 



The following table gives the general result of these experiments, arranged under 



* In these two experiments the percentage of iodide present cannot be given, as the weights of the specimens 

 were not noted. 



TRANS. ROY. SOC. EDIN., VOL. XLVI. PART I. (NO. 3). 



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