88 



D. NOEL PATON, B. P. WATSON, AND J. KERR ON 



(b) Proteins of the Fluids. — Transudations from blood-vessels contain the proteins 

 of the blood-plasma. In determining the source of the fluids, it was therefore necessary 

 to examine them for the presence or absence of such proteins. 



The qualitative examination was made by acidifying and boiling for the detection 

 of albumins and globulins, and by the addition of Esbach's solution for the detection of 

 lower proteins. 



The following table gives the results : — 



TABLE D. 







Allantoic. 



Amniotic. 



Sheep (61) 



+ 







Cow (9) 



+ 



+ 



Pig (7) 



+ 



+ 



Cat (2) 



+ 



+ 



Dog (1) 







+ 



Kestiakowski records the presence of albumin in the amniotic fluid of the cow 

 during the early stages of pregnancy only, and the presence of mucin in the later stages. 



The variations in the amount of proteins in the allantoic fluid throughout pregnancy 

 were studied in the sheep by precipitating either by boiling with addition of acetic acid, 

 or by the addition of an equal quantity of a 10 per cent solution of trichloracetic acid, 

 and weighing. 



The following table gives the results : — 



TABLE E. 



Weight of Foetus 



Per cent, of Protein 



Average 



in grms. 



in Allantoic Fluid. 



Percentage. 



2 



0-125 





10 



0-40 





10 



0-51 





12 



0-45 





13 



0-30 





13 



0-54 



0-42 



40 



0'89 





47 



0-33 



061 



200 



0-65 





390 



0-48 





150 to 650 



0-38 





400 



0-61 





400 to 800 



0-86 





600 



0-26 



053 



1500 



0-48 





1670 



046 



0-47 



