90 



D. NOEL PATON, B. P. WATSON, AND J. KERB ON 



(c) Non-Protein Nitrogen and its Distribution. — While the absence of native proteins 

 affords evidence of some value against the origin of the fluids by transudation, and while 

 their presence does not militate against the idea that the fluids are of renal origin, a study 

 of the presence of non-protein nitrogen, and the nature of the compounds in which it 

 occurs, should throw light on the relation of the fluids to foetal urine. 



The total nitrogen was determined in 5 or 10 c.c. by Kjeldahl's method. In the 

 allantoic fluids the total proteins were determined by precipitation with trichloracetic 

 acid, and the nitrogen either estimated directly or calculated from the weight of the pre- 

 cipitate. This was subtracted from the total nitrogen, and the non-protein nitrogen was 

 thus obtained. 



The urea nitrogen was determined generally by the method of Moener and Sjoquist, 

 sometimes also by the method of Bohland. By the former method a considerable 

 quantity of any allantoin present is estimated with the urea, and by the latter method 

 all is probably so estimated. The results of the earlier analyses were rejected, as the 

 amount of fluid taken (5 c.c.) proved to be too small to yield an adequate amount of 

 ammonia for accurate titration. 



Generally the urea results by Bohland's method were considerably higher than those 

 by Morner's, especially in the case of the allantoic fluids. 



TABLE G. 



Distribution* of Nitrogen in Amniotic and Allantoic Fluids. 



1 



Amniotic. 



Allantoic. 



















Per cent, of 



Weight 



Total 



M. and S. 



Per 



Total 



Protein 



Non-Protein 



M. and S. 



M. and S. to 



of Foetus. 



N. 



N. 

 •024 



cent. 



N. 



N. 



N. 



N. 



Non-Protein 



N. 



1-8 



•0368 



64 



■0688 







•0324 





5 



•0536 





... 



T37 



•093 



•044 







8 



•048 



•0312 



65 



T06 







•0312 





8 









T26 







■0524 





10 



•0256 



02 



78 



• • • 











10 



•0592 



•0424 



72 



T57 



•0816 



•076 



•0536 



70 



13 









•154 



•048 



T06 



•0704 



66 



13 









T71 



•086 



•085 



•0648 



76 



30 



•0256 



•02 



78 



•316 



T98 



T16 



•0592 



50 



32 



•0592 



•0508 



86 



T57 











35 



•0592 







•226 



•143 



•123 



•0536 



43 



41 



•0212 



•0156 



73 



T61 



•054 



T07 



•0744 



69 



150 



•066 



•055 



83 













195 



•048 







•328 



•101 



•227 



... 





196 



134 

















250 



14 



•101 



72 



•302 



■041 



•261 



•137 



52 



253 



■0648 



•062 















270 



132 

















328 



0368 



•0312 



84 



• • • 











370 



•06 



•0398 



66 



•332 



0976 



•235 



•15 



63 



390 



•076 







•276 



•0777 



T98 







