248 



DR JAMES A. GUNN ON 



Table III. — Minimum Lethal Dose by Subcutaneous Injection for Rabbits. 



No. of 



Weight of 



Dose per 



Actual Dose 





Experi- 



Rabbit 



Kilogramme 



in 



Result. 



ment. 



in Grammes. 



in Grammes. 



Grammes. 





14 



1120 



0-02 



0-022 



Recovery — slight effects. 



15 



1650 



0-08 



0132 



,, severe effects. 



16 



1150 



009 



0103 



>i )> >> 



17 



1000 



01 



01 



Death in 1 hour 30 minutes. 



18 



1450 



012 



0174 



„ 3 hours. 



Table IV. — Minimum Lethal Dose by Subcutaneous Injection for Rats. 



No. of 

 Experi- 

 ment. 



Weight of 



Rat 



in Grammes. 



Dose per 

 Kilogramme 

 in Grammes. 



Actual Dose 



in 



Grammes. 



Result. 



19 

 20 

 21 



22 



132 

 150 

 170 

 107 



0-076 

 0-09 



o-ii 



012 



o-oi 



0-0135 



0-019 

 0013 



Recovery — severe effects. 

 Death in 1\ hours. 



Table V. — Minimum Lethal Dose by Subcutaneous Injection for Cats. 



No. of 

 Experi- 

 ment. 



Weight of 



Cat 



in Grammes. 



Dose per 

 Kilogramme 

 in Grammes. 



Actual Dose 



in 



Grammes. 



Result. 



23 

 24 

 25 



2700 

 3000 

 1730 



0-09 



0-1 



013 



0-243 



0-3 



0-225 



Recovery— severe effects. 

 Death in about 9 hours. 

 „ \\ hours. 



For determination of the minimum lethal dose in frogs, and for all subsequent ex- 

 periments on frogs, the species Rana tem'poraria was used. In frogs, injections were 

 made into the dorsal lymph-sac, and in mammals under the skin of the right flank. 



Minimum Lethal Dose for Frogs.- — Recovery followed from doses of 0'2 gramme 

 per kilogramme and under; doses of 0'25 gramme per kilogramme and above proved 

 fatal. The minimum lethal dose is therefore about 0'25 gramme per kilogramme. 



Minimum Lethal Dose for Mammals. — In the cases of the guinea-pig, rabbit, and cat, 

 doses of 0"09 gramme per kilogramme and under were followed by recovery ; doses of 0'1 

 gramme per kilogramme and above proved fatal. The minimum lethal dose is there- 

 fore 0"1 gramme per kilogramme. In the case of the rat, recovery followed from doses 

 of 01 1 gramme per kilogramme and under, while 0"12 gramme per kilogramme was 

 the smallest dose to prove fatal. In this mammal the minimum lethal dose is therefore 

 slightly higher, namely 012 gramme per kilogramme. 



