250 DR JAMES A. GUNN ON 



At 6.30 p.m. no reaction resulted from either touching the conjunctiva or stroking 

 the nose. The eyelids were fully open, and the pupils semi-dilated. 



At tl a.m. next day, as also at 12.30 p.m., with the coil at 100 mm., stimulation 

 of the skin of either leg caused feeble movements of both legs, and stimulation of the 

 skin over the dorsal part of the cord caused fairly vigorous extension of both legs. 

 The circulation in the web of the foot was fairly active. 



At 3.20 p.m. stimulation of the skin of either leg with the coil even at 40 mm. 

 produced no movements of the opposite leg. Stimulation over the cord at 100 mm. 

 still produced feeble extension of both legs. 



The heart was now exposed by removing part of the sternum, and found to be 

 beating moderately strongly at the rate of 2 in ten seconds ; auricles and ventricle were 

 beating synchronously. 



The left sciatic nerve was exposed, and stimulation of it with the coil at 295 mm. 

 induced contraction of the left gastrocnemius muscle, but no movement of the opposite 

 limb ensued even with the coil at 40 mm. The muscles reacted to direct stimulation 

 at 50 mm. 



At 5.0 p.m. the heart was beating feebly at the rate of about 4 in 30 seconds. No 

 crossed reflex movements could be obtained by stimulation either of the skin of the leg 

 or the sciatic nerve. Stimulation over the cord with the coil at 80 mm. caused feeble 

 localised movements of the leg muscles, and stimulation of the sciatic nerve at 130 mm. 

 caused a feeble contraction of the gastrocnemius. 



At 10.0 p.m. the heart was found to be arrested in diastole, and the muscles and 

 nerves were irresponsive to electrical stimulation. 



(b) In Mammals. 



There is a close similarity in the effects produced by harmaline in different 

 mammals. To illustrate the general nature of these effects, the following two 

 experiments were selected ; the former as an example of the symptoms produced by a 

 minimum lethal dose, in the case of which death is postponed for several hours, the 

 latter as an example of a rapidly fatal dose. They also illustrate differences in the 

 symptoms in different animals. 



1. Guiriea-jngs. — Experiment 12. — Guinea-pig, male, weight 500 grammes. At 

 11.5 a.m. the cardiac impacts were 37, the respirations 21, in ten seconds. 



At 11.45, 0'05 gramme of harmaline hydrochloride dissolved in 4 c.c. Ringer's 

 solution was injected under the skin of the right flank. This was equivalent to 

 01 gramme per kilogramme. 



At 11.50 the cardiac impacts were 30, the respirations 18, in ten seconds. There 

 were slight tremors of the head, and the right hind limb was slipping on the tray. 

 During the next five minutes the animal made several short, jerky rushes forward ; in 

 the quiet intervals the hind limbs were extended as if unable to support the body- 

 weight. There were marked tremors of the head, and chewing movements of the jaws. 



