352 SIK THOMAS K. FRASER AND MR A.LISTER T. MACKENZIE ON 



were distended and the pupils large ; and the cardiac impacts were 6 per 10 seconds, 

 regular and shallow. In 5 hours 27 minutes, the throat respirations were 23 per 10 

 seconds ; the flank respirations were not visible unless the frog was disturbed ; the 

 three reflexes previously referred to were acute ; the muzzle rested on the floor of tray ; 

 the limbs were loosely flexed ; the frog could not jump well, the left anterior extremity 

 appeared to be powerless ; the pupils were widely dilated ; and the animal remained on 

 its back without any effort to recover the prone position, but when the abdomen was 

 stroked, the prone position was rapidly resumed. 



Nineteen hours after the injection, the throat respirations were 22 per 10 seconds; there 

 were no visible flank respirations while the frog was undisturbed ; the nose reflex was 

 dull, the conjunctival reflex almost gone, but the sacral reflex was acute ; when the 

 point of a blunt needle was lightly drawn across the ocular conjunctiva the frog breathed 

 more deeply and moved feebly, but there was almost no movement of the eyelids ; the 

 pupils were dilated and the limbs flexed ; when the posterior extremities were passively 

 extended, there was feeble twitching of the toes, but the limbs were not drawn up, even 

 on pressing the toes ; when the frog was laid on its back, the only movements resulting 

 were slight tremors of the toes, and, when the abdomen was stroked, contraction of the 

 muscles of the abdominal wall occurred ; the cardiac impacts were very distinct, and 3 

 per 10 seconds ; and the flanks were much distended. The frog now weighed 33 grams. 

 Twenty-four hours after injection, the conjunctival reflex was absent, and the condition 

 of the muscular system as last noted. 



Twenty-seven hours after the injection, the frog was lying flaccid; the conjunctival 

 reflex was present but sluggish ; the nose reflex and the sacral reflex were acute ; if one 

 of the posterior extremities was extended passively it was drawn up again, but only 

 after several efforts ; and the voluntary movements of the hind-limbs were feeble, and 

 accompanied by twitching of the toes. 



Two days after the injection, the throat and flank respirations were 20 per 10 

 seconds and regular ; the conjunctival reflex was acute ; the abdomen and flanks were 

 much distended ; and the frog could jump, but not well, and at each effort some urine 

 was ejected. Afterwards, the flanks became less distended, and the animal recovered 

 the prone position rapidly when laid on the back. It now weighed 32 grams. 



On the following day, the only symptoms were slight distension of the flanks and a 

 failure to jump as high as before the injection. The frog weighed 31 grams. 



Four days after the injection, the frog weighed 33 grams, and seemed in normal 

 health. 



The laboratory temperature was 57° F. 



Experiment XI. — 0*000084 gram of extract dissolved in Ringer's solution was injected 

 into the dorsal lymph-sac of a male frog (Rana temporaria) weighing 24 grams ( = 0*0035 

 gram per kilogram, or the minimum-lethal dose). Before the injection, the throat and 

 flank respirations were 28 per 10 seconds and regular in time ; the nose, conjunctival and 

 Sacral reflexes were acute ; and the posture was normal and the animal jumped well. 



