STROPHANTHUS SARMENTOSUS : ITS PHARMACOLOGICAL ACTION. 353 



Thirty minutes after the injection, the thorax was raised off the tray and the anterior 

 extremities were fully extended. One hour after the injection, the throat respirations 

 were 18 per 10 seconds and regular, while the flank respirations were 8 per 10 seconds, 

 and irregular in time and feeble ; the thorax was raised off the tray, with the fore-limbs 

 partly extended and widely abducted ; and the flanks were more distended than 

 previously. 



In 1 hour 25 minutes, the throat respirations were 19 per 10 seconds, and the flank 

 respirations 10 per 10 seconds ; the general attitude of the animal was as last noted; 

 and the flanks became more and more distended with each flank respiration, and, when 

 considerably distended in this way, they became flattened completely during a single 

 expiration. 



Five hours after the injection, the throat respirations were 20 per 1 seconds, feeble 

 and irregular, no flank respirations were visible and the flanks were permanently 

 distended ; the nose and conjunctival reflexes were acute, but the sacral reflex was less 

 acute ; the fore-limbs were flexed and slightly abducted ; the muzzle and throat were 

 raised off the tray and the thorax partly raised off; and the frog jumped well and 

 would not remain on the back. 



Nine hours after the injection, spontaneous active voluntary movements took place. 

 In 10 hours 30 minutes after the injection, the frog was quite motionless and flaccid, 

 and the pupils were contracted. When the heart was exposed, it was found to be 

 motionless and inexcitable, with the auricles large and dark and the ventricle small and 

 pale ; and a section of the ventricle gave an acid reaction to litmus paper. 



Twenty -four hours after the injection, the muscles of the upper part of the body, to 

 the level of the anterior extremities, were in rigor ; and stimulation of any part of the 

 surface with the secondary coil of a Du Bois Reymond's apparatus at zero, and a single 

 bichromate cell in the primary circuit, gave no response. Stimulation of the exposed 

 sciatic or direct stimulation of the muscles themselves with the secondary coil at zero 

 also elicited no response. The temperature of the laboratory during this experiment 

 was 56° F. 



Experiment XV. — 0*00017 gram of extract was injected into the dorsal lymph-sac 

 of a male frog (Rana esculenta) weighing 34 grams (equivalent to 0'005 gram per 

 kilogram, or ™ of the minimum-lethal dose). Before the injection, the throat and 

 flank respirations were 20 per 10 seconds and regular in time. 



Fifteen minutes after the injection, the throat and flank respirations were 24 per 

 10 seconds. In 30 minutes after the injection, the throat and flank respirations were 

 26 per 10 seconds, and the thorax rested on the tray and all four limbs were flexed. 

 In 50 minutes after the injection, the flank respirations were irregular in amplitude, 

 the nose and sacral reflexes were increased, the thorax was raised off the tray, and the 

 anterior extremities were extended. One hour after the injection, the throat respira- 

 tions were 8 per 10 seconds and regular, and the flank respirations were 8 per 10 

 seconds and irregular in amplitude. In I hour 12 minutes after the injection, the throat 



