STROPHANTHUS SARMENTOSUS : ITS PHARMACOLOGICAL ACTION. 355 



Two days after the injection, the rat was quite bright and active. On the third 

 day after the injection, the respirations were 24 per 10 seconds, the rat was active and 

 mentally alert when disturbed, but at other times it slept much. It now weighed 

 200 grams. 



No further symptoms were manifested. The temperature of the laboratory was 

 54° F. during this experiment. 



Experiment XXII. — 0'0975 gram of extract was injected subcutaneously into the 

 left flank of a female rat, of the weight of 130 grams ( = 075 gram per kilogram, 

 or 2 "5 times the minimum-lethal dose). Before the injection, the respirations were 

 18 per 10 seconds and regular, and the rat sat quietly in a corner of the tray. In 

 5 minutes after the injection, the rat ran round the tray, dragging its hind-limbs along 

 in the extended position ; the left hind-limb seemed paralysed ; thereafter the rat sat 

 down, and the posterior part of the body and the hind-limbs trembled violently for a 

 short time. During the next two hours no symptoms were manifested. 



In 2 hours 10 minutes after the injection, the respirations were 15 per 10 seconds 

 and regular, and the rat sat quietly. In 2 hours 35 minutes after the injection, the 

 respirations were 16 per 10 seconds, slightly irregular in time, and the head moved 

 gently with respiration ; the eyes were closed ; occasionally the head trembled 

 violently ; and when disturbed, the rat seemed dull, but it was able to run well. 



In 3 hours 45 minutes after the injection, the respirations were 14 per 10 seconds, 

 slightly irregular in time, and the rat was sleeping. In 5 hours 50 minutes after the 

 injection, the respirations were 16 per 10 seconds, noisy and regular; the rat was still 

 sleeping and showed no signs of uneasiness ; when disturbed, it ran round the tray as 

 if feeling rather than seeing its way ; and it was unsteady in its movements, and when 

 running it sometimes fell over on to the left hip. 



In 22 hours 40 minutes after the injection, the respirations were 16 per 10 seconds, 

 but sometimes the respiratory movement of the thorax was replaced by a rapid forward 

 movement of the head, which would occur three or four times in succession rhythmically 

 with the respirations ; and the rat was sitting drowsily in a corner with the eyes closed, 

 but when disturbed it ran about actively. In 26 hours 10 minutes after the injection, 

 the respirations were regular at the rate of 26 per 10 seconds, and the rat was asleep 

 placidly. 



In 46 hours 40 minutes after the injection, the respirations were 21 per 10 seconds 

 and slightly irregular in time ; and there was no evidence of discomfort. Fifty hours 

 40 minutes after the injection, the rat became very restless, sprang to the roof of its box 

 and gnawed the wire-netting ; this was repeated several times, and finally the rat sat on 

 the floor of the box breathing heavily. In 51 hours 40 minutes after the injection, 

 the respirations were 6 per 10 seconds, and both inspiration and expiration were 

 accompanied with loud wheezing ; and the rat sat with the eyes closed. In 53 hours 40 

 minutes after the injection, the respirations were 7 per 10 seconds, and were still 

 accompanied with noisy wheezing ; expiration was much longer than inspiration ; the 



