356 SIR THOMAS R. FRASER AND MR ALTSTER T. MACKENZIE ON 



cardiac impacts were strong, regular, and numbered about 36 per 10 seconds; and 

 voluntary movements were accompanied by well-marked tremors. Seventy-eight hours 

 after the injection, the rat was found dead and general rigor mortis was present. 

 When the thorax was opened, the lungs appeared to be quite healthy, the ventricles 

 of the heart were firmly contracted, and the auricles were dark and distended and 

 contained clotted blood ; the liver was healthy ; the stomach contained food ; the 

 cascum and large intestine were greatly distended with gas, and contained no 

 solid matter. The animal now weighed 120 grams. The laboratory temperature 

 was 50° F. 



Experiment XXIII. — 0*27 gram of extract was injected subcutaneously into the 

 left flank of a male rat weighing 180 grams ( = 1*5 gram per kilogram, or 5 times the 

 minimum-lethal dose). 



Before the injection, the respirations were 18 per 10 seconds and regular. One 

 hour 30 minutes after the injection, the respirations were 22 per 10 seconds, regular 

 but heaving in character ; the rat sat with the head lowered and the eyes almost closed, 

 and when disturbed it was not active ; and the cardiac impacts were masked by the heaving 

 respirations. Two hours after the injection, the respirations were 20 per 10 seconds 

 and there was much movement of the head and shoulders with respiration. Two hours 

 50 minutes after the injection, the rat became suddenly restless and tried to climb over 

 the edge of the tray. In 2 hours 55 minutes, the respirations were 18 per 10 seconds 

 and very laboured and heaving, and the eyes were completely closed. In 3 hours 35 

 minutes, the respirations were 18 per 10 seconds and expiration was prolonged and 

 accompanied by a hissing sound apparently produced in the nostrils, and occasionally 

 expiration was suddenly arrested for about five seconds. The rat was now lying with 

 the nose, mouth and thorax resting on the floor. In 3 hours 45 minutes after the 

 injection, the rat began to move about, but the head moved unsteadily and clonic 

 spasms occurred. It soon afterwards fell on the side and then walked with much 

 difficulty, dragging the thorax and abdomen along the floor. In 4 hours 12 minutes 

 after the injection, the respirations were 18 per 10 seconds and laboured; the muzzle, 

 throat, thorax and abdomen were on the tray, and when the head was raised it trembled 

 violently. In 4 hours 35 minutes after the injection the respirations were 6 per 10 

 seconds, deep and heaving. In 4 hours 58 minutes after the injection, several general 

 convulsions occurred and the animal died. 



(2) Rabbits. 



Experiment XXVIII — 0'00187 gram of extract was injected subcutaneously into 

 the left flank of a doe rabbit whose weight was 1870 grams ( = 0*001 gram per kilo- 

 gram, or § of the minimum-lethal dose). Before the injection, the respirations were 30 

 and the cardiac impacts 35 per 10 seconds and strong, the left pupil measured 5 mm. 

 transversely, the conjunctival reflex was acute, and the rectal temperature 38'5° C. In 

 30 minutes after the injection, the respirations were 21 per 10 seconds and the cardiac 



