358 SIR THOMAS R. FRASER AND MR ALISTER T. MACKENZIE ON 



the respirations were 30 per 10 seconds, the cardiac impacts 41 per 10 seconds and 

 easily felt ; the left pupil measured 6 mm. transversely ; and the rectal temperature 

 was 38° C. The rabbit weighed 1880 grams and seemed to have quite recovered. 



Experiment XXXVI. — 0*0043 gram of extract was injected subcutaneously into 

 the left flank of a doe rabbit whose weight was 2150 grams ( = 0"002 gram per kilogram, 

 or f of the minimum-lethal dose). Before the injection, the respirations were 32 per 

 10 seconds and regular; the cardiac impacts were 30 per 10 seconds, regular, but 

 not easily felt ; the transverse diameter of the left pupil was 7 mm. and the con- 

 junctival reflex was acute ; and the rectal temperature was 38° C. Ten minutes after 

 the injection, the respirations were 30 per 10 seconds and regular ; the cardiac impacts 

 were 40 per 10 seconds, regular and more easily felt; and the rabbit was restless and 

 there were occasional fine tremors in the muscles of the body and limbs. In 20 minutes 

 after the injection, the respirations were 35 and the cardiac impacts 34 per 10 seconds ; 

 the left pupil was unchanged ; the conjunctival reflex was acute ; the rectal temperature 

 was 37 '5° C. ; and the rabbit was more alert, but its back was less arched. Thirty minutes 

 after the injection, the respirations and cardiac impacts were each 30 per 10 seconds 

 and regular ; the left pupil measured 8 mm. transversely ; and the conjunctival reflex 

 was acute, and the skin reflexes over the posterior part of the trunk and the posterior 

 extremities were greatly exaggerated. Thirty-eight minutes after the injection, the 

 rabbit was very nervous and the panniculus carnosus muscle contracted over a wide area 

 when any sudden movement was made near the animal. Forty minutes after the injec- 

 tion, the respirations were 22 and the cardiac impacts were 35 per 10 seconds, and both 

 were irregular in time ; and fine muscular tremors occurred in the body and limbs. Fifty 

 minutes after the injection, the respirations were 26 per 10 seconds and irregular in 

 time, the cardiac impacts were 38 per 10 seconds and irregular in time and in force, 

 and the transverse diameter of the left pupil was 8 mm. One hour after the injection, 

 the respirations were 29 per 10 seconds and regular, the cardiac impacts were 46 per 10 

 seconds, regular and very distinct ; the left pupil measured 7 mm. transversely ; the rectal 

 temperature was 37° C. ; each respiration was accompanied by an abrupt movement of 

 the head ; and the animal did not refuse food. One hour 5 minutes after the injection, 

 the respirations were 26 per 10 seconds, and the cardiac impacts 46 per 10 seconds. 

 For about 15 seconds, the rabbit sat well back on its haunches with the back very con- 

 vex, the ears erect and the head shaking violently ; it pawed the floor with each anterior 

 extremity and thereafter it sank forward with the thorax on the tray and the cervical 

 portion of the spine very concave. One hour 10 minutes after the injection, the respira- 

 tions were 16 per 10 seconds and the cardiac impacts were 46 per 10 seconds, and 

 feeble ; the ears were quite erect ; and alternately the head was jerked upwards and then 

 sank slowly down. One hour 15 minutes after the injection, the rabbit raised itself on its 

 extended fore-legs and arched the spine strongly, a large amount of urine was then freely 

 passed, and thereafter it sank down and its head rested on the floor of the tray. One hour 

 17 minutes after the injection, the respirations were 4 per 10 seconds, but the cardiac 



