STROPHANTHUS SARMENTOSUS : ITS PHARMACOLOGICAL ACTION. 367 



solution up to the same point, viz. the level of the ankle-joint, and the left foot was 

 placed in the acid after the right had been withdrawn. The following experiment 

 illustrates the results obtained : — 



Experiment XL VI. — S. sarmentosus extract, 1 in 500. 



The left foot of a large frog was immersed for 5 minutes in a solution of the 

 extract in distilled water, 1 part in 500, to a point just above the level of the ankle- 

 joint ; and during the same time, the right foot was placed in distilled water to the 

 same level. Before immersion, the time which elapsed before withdrawal of either foot 

 from the acid was 5 seconds. This was tested on several occasions. One minute after 

 the immersion, the unpoisoned foot was withdrawn in 8 seconds and the poisoned foot- 

 in 7 seconds ; 8 minutes after immersion, the unpoisoned foot was withdrawn in 1 7 

 seconds and the poisoned one in 7 seconds ; 20 minutes after the immersion, the 

 unpoisoned foot was withdrawn in 15 seconds and the poisoned foot in 9 seconds. The 

 left foot was again immersed in the poison, this time for 10 minutes, the right foot 

 being meantime retained in distilled water. One minute after this immersion, the 

 unpoisoned foot was withdrawn from the acid solution in 22 seconds and the poisoned 

 in 18 seconds. Six minutes after immersion, the unpoisoned foot was withdrawn in 1G 

 seconds and the poisoned foot in 18 seconds; 1 hour 40 minutes after the immersion, 

 the unpoisoned foot was withdrawn in 23 seconds and the poisoned one in 26 seconds. 

 The left foot was again immersed in the poison, this time for 30 minutes ; and meantime 

 the right foot was kept in distilled water. Three minutes after this immersion, the 

 unpoisoned foot was withdrawn from the acid solution in 20 seconds and the poisoned 

 foot in 47 seconds ; 10 minutes after the immersion, the unpoisoned foot was withdrawn 

 in 15 seconds and the poisoned foot was not withdrawn in 2 minutes ; 25 minutes after 

 the immersion, the unpoisoned foot was withdrawn in 15 seconds and the poisoned one 

 was not withdrawn in 3 minutes ; 30 minutes after the immersion, the unpoisoned foot 

 was withdrawn in 10 seconds, whereas the poisoned foot was not withdrawn in 

 5 minutes. In the next observation, 40 minutes after the last immersion, the poisoned 

 foot was purposely placed in the acid solution to a point above the level of the contact 

 with the poison ; it was withdrawn in 28 seconds, while the unpoisoned foot was with- 

 drawn in 17 seconds; 50 minutes after the immersion, the unpoisoned foot was withdrawn 

 in 18 seconds and the poisoned foot in 60 seconds — only the poisoned portion was in 

 contact with the acid solution ; 55 minutes after the immersion, the unpoisoned foot was 

 withdrawn in 16 seconds and the poisoned one in 80 seconds; 70 minutes after the 

 immersion, the unpoisoned foot was withdrawn in 15 seconds and the poisoned foot 

 in 25 seconds ; 90 minutes after the immersion, the unpoisoned foot was withdrawn in 

 15 seconds and the poisoned foot in 30 seconds ; 2 hours after immersion the reaction 

 time was 16 seconds for the unpoisoned foot and 30 seconds for the poisoned foot ; 

 4 hours 10 minutes after the immersion, the times were 28 seconds for the unpoisoned 

 foot and 40 seconds for the poisoned foot; and 21 hours after immersion, each foot wag 

 withdrawn from the acid solution in 45 seconds. 



