34 CIRCULAR 3 9 2, U. S. DEPARTMENT OF AGRICULTURE 



sample in a wooden (fig. 21) or strong cardboard box. Do not use 

 tin, glass, or waxed paper. 



Smears of dead brood and small crushed pieces of comb are fre- 

 quently unsatisfactory for diagnosis but will be examined in case 

 the foregoing instructions cannot be followed. 



HOW TO SEND SAMPLES OF ADULT BEES 



(1) Select, if possible, bees that are sick or recently dead; bees 

 that have been dead for some time are not satisfactory for examina- 

 tion. (2) Send at least 50 bees in a sample; if poisoning by ar- 

 senicals is suspected, 200 or more bees will be needed for analysis. 

 (3) Send bees in a wooden or strong cardboard box and not in tin 

 or glass. 



£Z°^ 



e > MO C£/Vr £# 



Figure 21. — How to send a sample of brood for laboratory examination. 

 HOW TO SEND SAMPLES OF TREATED COMB 



(1) Send a sample not less than 4 by 5 inches in size if in- 

 fection is heavy, or an entire brood comb if infection is slight. (2) 

 Brood remains should be present in abundance. (3) Pack the comb 

 in a clean wooden box as soon after treatment as possible. (4) 

 Do not send samples that contain honey. 



HOW TO ADDRESS SAMPLES 



All samples should be addressed to the Bee Culture Laboratory, 

 Bureau or Entomology and Plant Quarantine, National Agri- 

 cultural Eesearch Center, Beltsville, Md. 



Your name and address should be plainly written on the box. 

 If the sample is forwarded by an inspector, his name and address 

 should also appear on the box. 



