918 



SIR THOMAS R. ERASER ON THE 



Among these experiments, also, attention may be directed to the series of three 

 experiments made on the same day (12th October 1914), in which with a dose of 

 0'025 grm. per kilo much blood was found in the large intestine and only a little in 

 the stomach and small intestines ; with a dose of 0'05 grm. per kilo the extravasated 

 blood was more equally distributed throughout the alimentary canal ; and with a 

 dose of 0*1 grm. per kilo much the greater part of the blood was present in the 

 stomach. These facts suggest that the elimination of the haemorrhage-producing 

 constituent of the poison occurs chiefly if not entirely into the stomach, a suggestion 

 that receives support from some also of the experiments in which the arrow-poison 

 was injected under the skin of a thigh (Table XVI, experiments with 0'05 and 

 O'l grm. per kilo). 



(c) Ether Extract from Poison of Abor Arrows from the Government of India. 



For the purpose of isolating this constituent, the arrow-poison was extracted 

 with water, weak acids, alcohol, and ether ; and, although each product caused effects 

 similar to those caused by the arrow-poison, the most satisfactory product was 

 obtained with ether. A small quantity of the poison was extracted in a Soxhlet 

 apparatus with pure ether (720), and on evaporating the ether a substance re- 

 presenting 21 '8 per cent, of the dried arrow-poison was obtained. It was a slightly 

 viscous fluid, of a brownish-yellow colour, and gave an oily stain to paper. 



When 0'0024 grm. of this ether extract was injected under the skin at the flank 

 of a rat weighing 235 grms. (representing O'Ol grm. per kilo), no other effect was 

 produced but a local swelling at the site of injection, which appeared within 24 hours 

 and had not altogether disappeared one month afterwards. 



In frogs, however, besides local effects, the same general and lethal effects were 

 produced as by the arrow -poison itself. The experiments with this animal are 

 summarised in Table XVIII. 



Table XVIII. — Ether Extract from Poison of Abor Arrows from the Government of India. 

 Frogs. — Injection into the lower dorsal lymph space. 



Date. 



1914 

 Mar. 29 



29 



"Weight 

 of 



Animal. 



Dose 

 per 



Kilo. 



Actual 

 Dose. 



Result. 



Notes. 



25 



31 



000025 

 0-0005 



0-00000775 

 0-0000155 



Recovery. 

 Recovery. 



No symptom observed until 7 days, when a 

 considerable quantity of blood was passed 

 per ano, and traces continued to be passed 

 occasionally for the next 30 days. 



No symptom observed until 7 days, when a 

 considerable quantity of blood was passed, 

 and this was repeated with varying 

 quantities on the 10th, 12th, 14th, 17th, 

 26th, and 35th days. 



