920 



SIR THOMAS R. FRASER ON THE 



As, therefore, this ether extract, representing about one-fifth of the weight of 

 the arrow-poison from which it had been derived, reproduced the effects of that 

 arrow-poison, with a lethal power in frogs at least five times greater, it may be 

 regarded as the active ingredient. 



With the purpose already explained, two series of experiments with this ether 

 extract, also, were made on frogs, in one of which it was injected under the skin of 

 a thigh, and in the other of a leg below the knee. 



Their results are displayed in Tables XIX-XXI. 



Table XIX. — Ether Extract from Poison of Abor Arrows from the Government of India. 

 Frogs. — Injection under the shin of a thigh or of a leg. 



Date. 



Weight 



of 

 Animal. 



Dose 



per 



Kilo. 



Actual 

 Dose. 



Result. 



Notes. 



1914. 

 June I 



Injection under the skin of a thigh. 



29 



May 5 



20 



20 



0-025 



22-5 



0-05 



0-01 



0-000725 



0001 



Death in from 

 2 to 3 days. 



Death in about 

 24 hours. 



No change in rate of cardiac or respiratory 

 movements. On 2nd day, motor weakness ; 

 and on 3rd day, passage of blood-stained 

 gelatinous substance per ano. After death, 

 blood-stained fluid under skin of trunk ; heart 

 dilated ; much blood in stomach and small 

 and large intestines. 



Do. do., except no blood passed. After death, 

 as in previous experiment. 



o-oi 



Injection under the skin of a leg below the knee. 



0-0002 Death in from On 2nd day, motor weakness. Skin of injected 

 5 to 6 days. leg purplish red from knee to ankle, and on 



3rd day of lower thigh and foot. After 

 death, several large and punctate haemor- 

 rhages in tissues of injected leg and thigh 

 and of sacral region, and small linear haemor- 

 rhages in upper thigh of opposite leg. Blood 

 in stomach and large intestines. 



0'000225 Death in less Injection made in ventral aspect of leg. No 

 thanl8hours. symptom observed. After death, slight 

 pallor of tissues everywhere. Muscles con- 

 tracted on galvanic stimulation of sciatics, 

 but no reflexes. Cardiac ventricle pale in 

 medium diastole. Skin over dorsal aspect 

 of left leg slightly darker than over right 

 leg ; no difference in ventral aspects. Below 

 the skin, in neither leg nor thigh was there 

 any congestion or haemorrhage, nor in any 

 subcutaneous or deep tissue anywhere in the 

 body. Stomach greatly enlarged, bluish 

 purple, and blood-vessels dilated. Small 

 intestines almost empty ; large intestine 

 moderately large and slaty blue. In stomach, 

 dark red gelatinous substance which gave 

 spectroscopic and colour reactions of blood, 

 and which contained numerous blood cor- 

 puscles. Reactions of blood, but no blood 

 corpuscles, in contents of small and large 

 intestines. No haemorrhages or congestion 

 of brain or spinal cord. 



