84 DR JAMES A. GUNN ON 
desirable also of the pharmacology of harmine, especially as there seems some prospect 
of the alkaloids being of therapeutic value. 
Iam much indebted to Dr J. F. Tuorps, F.R.S., for his great kindness in giving 
me several grammes of pure harmine for pharmacological investigation. From the 
base I prepared, according to his directions, the hydrochloride of the alkaloid, and with 
this salt all the experiments to be described were performed. 
A. LetHauity or HARMINE. 
The lethality of harmine was determined for frogs, guinea-pigs, rabbits, rats, and 
pigeons, with the following results :— 
Taste I.—Mintmum Letuat Dose sy SuscuTaneous INJECTION FoR FROGS. 
No. of Weight of Dose per Actual Dose 
Experi- Frog Kilogramme in Result. 
ment. in Grammes. in Grammes. Grammes. 
1 18 0°4 0:0072 Recovery. 
2 20 0:5 0-01 * 
3 16 06 0:0096 Death in about 6} hours. 
4 23 08 0:0184 as a > 
Tasie IJ.—Minimum Letuat Dost sy SuscutTaNneous INJECTION FOR GUINEA-PIGS. 
No. of Weight of Dose per Actual Dose 
Experi- Guinea-pig Kilogramme in Result. 
ment. in Grammes. in Grammes. Grammes. 
5 620 0°08 0-05 Recovery. 
6 750 01 0:075 5. 
7 700 0-12 0-084 Death in 2 hours. 
TasLe IIJ.—Minimum Letuat Doser sy SuscuTangous INJECTION FOR RaBssits. 
No. of Weight of Dose per Actual Dose 
Experi- Rabbit Kilogramme in Result. 
ment. in Grammes, in Grammes. Grammes. 
8 1200 0°15 0°18 Recovery. 
9 1750 0-2 0°35 a 
10 1600 0:23 0'368 Death in 2 hours 40 minutes. 
11 1550 0°3 0°465 » lL hour 12 minutes. 
