THE PHARMACOLOGICAL ACTION OF HARMINE. 85 
TasLe [V.—Minimum Leruay Dose sy SuscutTaneous INJECTION FoR Rats. 
No. of Weight of Dose per Actual Dose 
Experi- Rat Kilogramme in Result. 
ment. in Grammes. in Grammes. Grammes. 
12 155 0-1 0:0155 Recovery. 
13 100 0°15 0015 5 
14 150 0:2 0:03 5 
15 100 0°2 0°02 Death in 3 days. 
16 115 0:3 0:035 », 9-20 hours. 
17 115 0-4 0046 ye, f=20' 5 
Taste V.—Minimum Letuat Dose sy SuscuTaNgzous INJECTION FOR PIGEONS. 
No. of Weight of Dose per Actual Dose 
Experi- Pigeon Kilogramme in Result. 
ment. in Grammes. in Grammes. Grammes. 
18 300 01 0:03 Recovery. 
19 430 0:12 0-051 3 
20 | 420 0-15 0:063 Death in 10 minutes. 
| 
For determination of the minimum lethal dose in frogs, and for all subsequent ex- 
periments on frogs, the species Rana temporaria was used. In frogs, injections were 
made into the dorsal lymph sac, in mammals under the skin of the right flank, and in 
pigeons under the skin of the right thigh. 
From the above tables it is seen that the minimum lethal dose by subcutaneous 
injection per kilogramme is, for the frog, 0°6 gramme ; for the guinea-pig, 0°12 gramme ; 
for the rabbit, 0°23 gramme ; for the rat, about 0°2 gramme; and for the pigeon, 0°15 
gramme. 
B. SYMPTOMS PRODUCED BY HARMINE. 
(a) In Frogs. 
Experiment 3.—Rana temporaria, male, weight 16 grammes. At 12.30 p.m. the 
throat respirations were twenty in ten seconds and the cardiac impacts seven in ten 
seconds. 
At 12.40, 0°0096 gramme of harmine hydrochloride dissolved in 0°48 c.c. of saline 
solution was injected into the dorsal lymph sac. This was equivalent to 0°6 gramme 
per kilogramme. 
At 1.40 the respirations were feebler and somewhat irregular, the average rate 
being about 14 per ten seconds. The cardiac impacts were five in ten seconds and less 
distinct than before injection. The back was stiff owing to rigidity of the back 
muscles. The frog was unable to jump, and turned over with difficulty when placed on 
