86 DR JAMES A. GUNN ON 
its back, an effect partly due to some stiffness of the thigh muscles caused by diffusion 
of the injected solution. The conjunctival reflex was sluggish, and there was 
some impairment of the reflex excitability of the cord as determined by electrical 
stimulation. 
At 2.30 the frog was unable to jump, and could not recover the ventral posture when 
laid on its back. 
At 8.0 the respirations had ceased and the cardiac impacts were not visible; but, 
when the web of the foot was examined under the microscope, the blood was found to 
be circulating sluggishly. Stimulation of the skin of one leg produced no movements 
of the opposite leg, even with the coil at 30 mm. ; but stimulation of the skin over the 
sciatic nerve produced a contraction of the gastrocnemius muscle of the same side, with 
the coil at 120 mm. | 
At 4.30 the circulation was found to be arrested in the web. The brain was pithed 
and the heart exposed. ‘he heart was beating feebly at the rate of 2 in ten seconds. 
It ceased beating in ten minutes. No reflex movements could be elicited even by direct 
stimulation of the sciatic nerve with the secondary coil at 20 mm., but contraction of 
the gastrocnemius muscle of the same side was induced by stimulation of the nerve at 
100 mm. 
(6) In Mammals. 
EKexperiment 11.—Rabbit, 1550 grammes. At 10.55 a.m. the cardiac impacts were 
46, and the respirations 34, in ten seconds. The temperature was 99° C. 
At 11.0, 0°465 gramme dissolved in 10 c.c. of warm saline solution was injected 
under the skin of the right flank. This was equivalent to 0°3 gramme per kilogramme.., 
At 11.5 there were marked tremors of the head and fore part of the body, and the 
hind limbs were extended so that the abdomen touched the ground. Three minutes 
later the tremors were more violent and the animal made frequent spasmodic movements 
forwards, the hind limbs being unable properly to support or propel the body. 
At 11.12 a slight epileptiform convulsion occurred, marked especially by clonic 
movements of the limbs, after which the animal lay quiet. A similar but more violent 
convulsion occurred a minute later, during which the animal fell on its side and rolled 
over sideways two or three times. After this the rabbit lay on its side with feeble 
pawing movements of the limbs. The respirations were 20, and the cardiac impacts 
35, in ten seconds. The temperature was 100° C. 
Up to 11.30 the symptoms were similar, but the convulsive movements became 
gradually less violent. At the end of that time the respirations were 12 in ten seconds, 
regular and deep. The cardiac impacts were palpated with difficulty, and were about 
28 in ten seconds. The skin was colder. 
At 11.50 there was almost complete motor paralysis, the animal lying continuously 
on its side and making feeble running movements occasionally. When held up by the 
ears it made no movements. Pinching the skin evoked no reflex movements, and the 
conjunctival reflex was sluggish. 
