1903.] "THREE SPECIES OF MAHINE EGBALLOCYSTIT, 201 



describe one of the new species with the name Ecballocystis Willeana 

 to commemorate the kind advice of Prof. Wille bestowed on me. 



Ec, Willeana is an inhabitant upon a more or less smooth rocks at 

 high water mark. It forms dark green fleckles on the substratum. At the 

 beginning of the development, the thaUus is solid and flat and more or 

 less wrinkled at the upper surface and adheres to the rock by the lower 

 surface, (fig. 2). As the plant grows larger the surface of the thallus 

 becomes much more wrinkled giving an appearance of highly confused 

 tuberculation (fig. 1). Cutting the thallus longitudinally we find hollow 

 empty room within the tuberous portion with the cells radially directed 

 toward the external surface. These cells are densely aggregated at the 

 periphery and rarified at the interior. The peripheral cells are club shaped 

 with the thicker ends directed toward the periphery and might be pro- 

 visionary divided into two compartments. The upper compartment is spaceous 

 with chromatophore lining the whole part and a large spherical pyrenoid 

 at the center. The lower compartment is narrow, destitute of chromatophore 

 but often with a vacuole and minute granular bodies ; it tapers downward 

 ending finally in a sharp point (fig. 10). The cells near at the substrat- 

 um are globular in shape and are enveloped within a sort of very thick 

 gelatinous membrane (fig. 24). They give rise to a process toward the 

 base which elongates further forming a colourless cylindrical filament until 

 it had reached substratum where it serves as an adhering organ (fig. 6). 



Zoosporangia (Gametangia ?) are evoluted directly from the vegetative 

 cells. The cell content divide successively until we have 6-18 or often 

 more zoospores in a sporangium, differing in their number aqcording to the 

 size of the mother cells. The evolution takes place in the internal cells 

 at first and in the peripheral cells at last. Each zoospore has a conspicuous 

 pyrenoid at a corner. 



A thin layer of starch is found enveloping the pyrenoid of the vege- 

 tative cells as well as zoospores (figg. 10. ll. 13). When we treat the 

 object with strong caustic potash for a few minutes, wash in water 

 thoroughly and then stain with the iodine solution the reaction is most 

 remarkable. In the section thus prepared we find that the peripheral cells 

 have the starch layer around the pyrenoid only, while in the internal cells 

 the minute starch grains fill up the whole parts of the upper compartments ; 

 in most cases we can not recognize, in the latter, the position of the 

 pyrenoid as it is now entirely covered with dark bluish granules, but in 

 the cells at the transition stage from the peripheral to the internal we 



