202 ' FLOBUBA TSUSIMJBNSIS. [Vol. xvii. 



can distinguish the globubar body by the deeper staining (figg. 14. 15). 

 The grains in the rhizoid cells are localized in two or three places. The 

 greater portion is around the pyrenoid which is now often hard to detect, 

 and some portion at the tip or near at the tip of the process. Often 

 another portion is found in the midway of the process. 



Careful study of the thallus shows that it is practically formed of a 

 group of the colonies of cells combined together within a slimy matrix 

 Each cell is stored within a hyalin gelatinous membrane much denser in itg 

 substance than the surrounding matrix. A cell divides along an oblique 

 plane through the middle part of the upper compartment. One of the 

 resulted cells travels diagonally upwards leaving a part of the membrane 

 behind in a form of gelatinous stalk^^to the cell; then it divides again 

 repeating the similar process as before, thus finally forming a colony of 

 several cells. Usually the process repeats more rapidly in one side of the 

 colony. The result is some of the cells remain deep in the interior of the 

 thallus while some have attained to the periphery (fig. 7). 



The gelatinous stallcs of the cells are easily perceived under a high 

 powered microscope in a meridional section of the thallus. Very often we 

 find a stalk with the transverse concentric zones of dark and hyaline layers 

 with their centers along the axis of the stalk. It is quite certain that these 

 zones should have the similar meaning with those found at the rear of the 

 Eagelonopsis cells^^ (fig. 9). 



For the closer obesrvation of the branching mode of the stalk, lowever, 

 it is better to treat the sections with a staining reagent. I found methyl- 

 green answers best for the purpose; and also chloriodide of zinc in like 

 manner. The former stains the gelatinous membrane and the stalk in a 

 bright green, and the latter in a beautiful purple, while the surrounding 

 matrix remains for a greater part unstained (fig. 7). 



In the preparations thus stained the present writer failed to find the 

 starting point of the branching stalks. Even in the branches stained in 

 the utmost degree their lower portions have been always obscure and finally 

 unsparable from the surrounding matrix. It can be said with utmost 

 certainty that the older portions of the branches become degradade^ and 

 confluent with the gelatinous substance which combiness the colonies 

 together. 



Ec. ja'ponica is closely related to the preceeding species in the external 

 as well as in the internal characters. But in most cases the thallus is 



1) Davis : Euglenopsis. p. 382. (Annals of Bot. VIIL 1894.) 



1 



