1903.] THREE SPECIES OF MARINE ECBALLOCYSTIS. 203 



much flattened in this species and the cells smaller in general. The cells 

 at the periphery are oblong, more or less angulate and highly variable in 

 their size. The gelatinous stalks are extremely short and not easily 

 recognizable before the treatment. To obtain, a clearer view of the branch- 

 ing of the stalks I recommend to boil the section in 50^^ hydrochloric acid 

 for a few minutes, and after washed throughly in water the colouring 

 reagents before mentioned should be applied on. When thus treated the 

 surrounding matrix becomes dissolved in a measure, the colonies separated 

 from each other, and the characteristic branching mode is now visible 

 (fig. 17). The stalks are, however, much shorter than in the preceeding 

 species. The cells at the interior part of the thallus are mostly globular 

 but very often 2-4 cells are found in a group enveloped in a common 

 gelatinous capsule and mutually pressing. 



The chromatophore lines the whole or nearly the whole part of a cell. 

 A large pyrenoid is at the center of the cell and the starch layer is found 

 covering it as in the former species. A dark brownish, highly refracting 

 granule and one or two vacuoles are found at one corner of the cell. 



Propagation is not known in the present species. 



Ec. cava has bright green thallus and appears like a young plant of 

 Viva in its habit. It attains the largest size among the genus. The first 

 course of the development is like Ec, Williana but soon it becomes much 

 inflated, hollow inside and situated at the surface. The largest diameter 

 of the bulbose specimens measured 19mm. 



In a longitudinal section of the thallus we do not find the radial 

 arrangement of the cells which has been the case in the Canadian alga. 

 In "this species as well as in the preceeding the cells are much densely 

 aggregated at the periphery and quite irregular at the interior (fig. 21). 

 The peripheral cells are mostly hemispherical and generally two cells fVice 

 against one another at the flattened side. (fig. 23). The internal cells are 

 globular and stored in a thick gelatinous capsel (fig. 24). Each of these 

 cells when situated near at the substratiun gives rise to a process down- 

 ward through the gelatinous matrix. The cell contents translocate en 

 mass into the process as it elongates further below, assuming a rod-like 

 form, and leaving the original cell room filled with a gelatinous substance 

 apparently inseparable from the capsular membrane (fig. 22). In Ec. 

 Willeana we could not find the translocation of the whole cell contents 

 and in Ec, japonica a few cases as this have been met (flg. 19). In the 

 present species, however, the long colourless filaments traversing through 

 the gelatinous matrix are highly developed and not only the basal cells but 



