204 BOTANICAL MAGAZINE. [Vol. xvii. 



also the cells deep in the thalhis share the formation of the filaments* 

 Some times we find concentric zones of the gelatinous layers in the original 

 cell room. The case has been also met in the preceeding species (fig. 19). 



Chromatophore lines the half part of a cell. The pyrenoide and the 

 starch layer as in the preceeding species. In the rhizoid cells of the pre- 

 sent species, however, the localization of the starch grains is not similar as 

 in those of Ec. Willeana. The pyrenoid assumes the middle position in 

 the rod and the starch is found exclusively surrounding it (fig. 23.) 



Zoospores (Gra metes ?) are formed in the cells of the thallus by succes- 

 sive division of the cell contents as in Ec. WiUeana. 



The characteristic gelatinous stalks are very hardly recognizable in the 

 present species. On boiling in 50^^ hydrochloric acid as before the matrix 

 becomes dissolved and a number of colonies consisting of dozen or more 

 cells are resulted. The cells in a colony are closely combined and seem as 

 if imbedded in a common gelatinous envelope. But often we find a young 

 stage of the colonies in which the stalks are comparatively longer and the 

 view of the characteristic branching mode is easily caught. Chloriodide of 

 zinc answers better in this case (fig. 25). 



Ecballocystis is related to Euglenopsis, Prasinocladus and Ghlorangium 

 as Bohlin has already mentioned^^ Ec. Willeana shows especially closer 

 afiinity with Prasinocladus ; and Ec. cava should stand at the remotest 

 position among the members of Ecballocystis in consideration with the 

 gelatinous stalks and the compactness of the colonial union. 



Lately Setchell and Gardner^^ have jointly established a new genus 

 ColUns^ella by a plant collected at Whidby Island which lies not far from 

 Port Eenfrew. It seems to me highly probable that Collinsiella tuhercu- 

 lata, Setch. et Gard. would be a young and sterile form of a plant standing 

 near by Ec. Willeana, if not the same species. They delineated the 

 branches of the gelatinous stalks extremely clear, almost to the starting 

 point (PI. 17. fig. 3 1. c.) In our plants, however, as has been already 

 observed, the branches were not very far clearer than shown in the accom- 

 panying plate (figg. 7. 17. 25), although trials have been repeatedly done 

 on each species. 



If Setchell and Gardner's plant shows the branches as clearly as they 

 have given, it undoubtedly claims the extreme position among Ecballocystis, 



1) 1. c. p. 8. 



2) Algae of Northwest America, p. 204. 



