446 General Notes. [ April, 
Gage’, who had begun to experiment with collodion before 
Schallibaum’s method was published, recommends that the collo- 
dion and clove oil be applied separately: 
“A solution of collodion is prepared by adding to 2 grams of 
gun cotton (that used by photographers is good) 54° of sul 
phuric ether and 18° of 95 p. c. alcohol. After the gun cotton 
is entirely dissolved, the solution should be filtered through 
ter paper or absorbent cotton. The slides are coated by pour 
ing the collodion on one end, allowing it to flow quickly over the — 
slide and off the other end into the bottle. The prepared slides — 
should be kept free from dust. As the collodion will not deter- 
orate after drying on the slide, any number of slides maybe 
prepared at the same time. Before using a slide it shouldbe 
dusted with a camel’s hair brush, and with another brush the — 
collodionized surface of the slide should be thinly painted with 
clove oil. | 
“x * * The sections are arranged as in the shellac method. 
The slide is warmed over an alcohol lamp and then heated ae. 
warm chamber so as to drive off the clove oil. After cooling," 
mass within half an hour, though the slide may remain in any he 
without loosening the sections. When the slide is rena 
from the naphtha, the sections are washed with 95 P-© ert in 
by means of a medicine-dropper, or by immersing the A a 4 
alcohol. If the sections are to be stained in Kleinenberg $ ithe 
toxylin or inany other stain containing 50 p.c. Or reg pete 
0 
ie Ae divin: t transferred to § ; 
e staining agent, otherwise it should be firs W enever the . 
and finally in 95 p.c. alcohol to completely anhydrate the ge i 
T ey are cleared with a mixture of carbolic acid 1 prei “o 
tine 4 parts. The balsam to be used is prepared by 
grams of pure Canada balsam with 2” of chlorolo 
clove oil. The latter very soon removes any cloudiness 
have appeared in the collodion film.” 
a 
Born’s METHOD or RE-CONSTRUCTING One pes 
scopic Secrions?— Dr. G. Born describes in deta 3 al 
l os» The Medic 
H. Gage and T. Smith. “Serial Microscopical Sections,” Zhe Me 
P- 14, November, 1883. : xxi 
2G. Born. “ Die Plattenmodellirmethode.” Archiv. f. Mik- Anat. 
1883. First described in Morphol. Jahrbuch 11., p. 578, 1879 
poe 
