556 General Notes. | May, 
most kindly presented the whole collection on exhibition to the 
useum of Archzology at Cambridge, Mass., and since its arri- 
val I have again examined the specimens, and have no hesitation 
in pronouncing a large proportion of them as the work of man. 
Some are simple chips or flakes, split off in the process of work- 
ing the stones into shape. Others are pointed pieces of quarts 
which may in part be natural fractures, but which have received a 
few finishing blows from the hands of palzolithic men. A few 
others, including one or two not of quartz, are evidently natural 
forms, but in the collection are about a dozen unquestionably 
chipped implements, which, except that they are made of quartz 
instead of argillite, closely resemble the palzolithic implements 
found by Dr. Abbott in the New Jersey gravels. For the present 
I refer to Miss Babbitt's paper, now in course of publication by 
the American Association for the Advancement of Science for 
further details, but I hope at no very distant day to give illustra- 
tions of these specimens in our reports, as the museum has now 
become their owner.—/. W. Putnam. 
MICROSCOPY AND HISTOLOGY." 
METHODS OF STUDYING THE SO-CALLED potas sig Haut pe 
cEA.*—For the study of fresh tissues Dr. Frenzel placi 
piece of the organ pd the slide, zz the blood of the individna ES 
which it was taken ; or, in sea-water diluted until the salt pv i 
amounts to about 114-2 per cent (one part distilled pepe s 
part sea-water from the Bay of Naples). The so-called “ p i 
logical salt solution” (34 per cent) worked unfavorably, © 
maceration. fee : partly 
Various fluids were employed for killing and hae and 
for determining the effect of different reagents on the F orepating 
the protoplasm, and partly for finding the best means 0! p 
the object for sectioning. ‘ alcohol from 
ery good preparations were obtained with warm , gua n in 
seventy per cent to ninety per cent; while direct 1m vik 
absolute alcohol did not prove advantageous. thie the struc- 
gave good results for the cell protoplasm, but destroy for the cells 
ture of the nuclei. Still better results were obtained to se 
(not for the nuclei) by adding a few drops of iodine 
per cent alcohol. mmersing the : 
blimate from 
The most satisfactory results were reached by ! 
object in a saturated aqueous solution of corrosive cae 
ten to thirty minutes, then washing with water, an 
ing the water gradually with alcohol. - ved with corrosivé 
erenyi’s fluid gave best results when combine minutes i 
sublimate. The object was left from five to ten 
1 Edited by Dr. C. O. WHITMAN, Mus. Comparative Zoology, Cam 
`~ * Johannes Frenzel. ‘ Ueber die Mitteldarmdriise der Crustaceen 
Zool. Station, v, p. 51, 1884. 
pridge, MP 
” mitthell.* 
ERE SP E RA cen ee be oom ND E ea 
