Preliminary Note on the Cells of Vicia faba 



modified by Rontgen Rays and their 



Resemblance to Tumor Cells 1} . 



By 



Hideo Koimiro. 



I experimented on the effect of Rontgen rays upon the mitoses 

 of the cells of radicles of Vicia faba in 1916 and the results were 

 published in 1917 2) . At that time I paid my attention chiefly to 

 the changes of chromosomes and made no careful observations on 

 other cell elements. 



The material of present work was the one which was used for 

 physiological experiments performed on April 19—27 1919. That 

 was the seeds of "Hyogo", a race of Vicia faba, which were steeped 

 in water for 77 hours (water content reached 57.87^) and exposed 

 to the rays of 20H, 40H and 50H on April 19, and sowed in sands. 

 All the seedlings irradiated reached on April 27 nearly the same 

 stage of growth, as those shown in Fig. 3 of my paper in this 

 magazine published in Oct. 1920. The tips of radicles of Vicia faba 

 irradiated at different intensities together with the controls were 

 fixed in Flemming's fluid at noon of April 27 1919. 



The sections were cut in 6— 10/i, and stained with iron- 

 haematoxylin 3) or Flemming's orange process 3) or iron-haematoxylin 

 with the counters! ain of Congo red. 



In control preparations, binucleated cells of almost equal sizes 

 were frequently observed in the plerom tissue. In long cells of this 

 part both the nucleus and nucleolus become elongated — sometimes the 

 latter takes an irregular shape and vacuolated. Some have two 



1) I have much pleasure in acknowledging here my indebtedness to Prof. 

 Tetsuji Kimura who has permitted me to compare his tumor preparations with 

 mine and given me valuable informations on tumor problem. 



2) On the effect of Kontgen rays upon the cell and tissue of Vicia faba L. 

 "Irigaku Kyoho Zassi " (The Journal of Physical Therapy) No. 6, Aug. 1917. 



3) Meyer, A. : Erstes mikroskopische Praktikum. 3. Aufl. 1915. pp. 200-202. 



