Parker.] 100 : [November 6, 
alone. These were kept at a temperature of 100° Cent. (212° F.) for 
thirty minutes. The parsnip infusions were opened on the sixteenth, 
twentieth, and twenty-second days. Nothing was found in the de- 
posits except granular matter. The flasks containing urine with 
liquor potass. added, were examined on the tenth, fifteenth, and 
nineteenth days after sealing, with the same result. The flasks con- 
taining the juice of beef were opened in eight and ten days after 
closing, and the infusions were found to exhibit fat globules only. 
The flasks containing simple urine were examined on the tenth and 
seventeenth days after sealing. They showed not a vestige of any 
organism. 
On April 9th, a third lot of twelve flasks were prepared and 
sealed, viz.: four of hay, three of green clover, three of turnip, and 
two of parsnip. To these nothing was added. ‘They were kept at a 
temperature of 120° Cent. (248° F.) for one hour. On the fourth 
day, one of the flasks containing the infusion of turnip was opened. 
The other two remained closed until one hundred days had elapsed. 
The deposits were found to consist of granular matter. The flasks 
containing hay infusions, were opened on the twenty-first, forty- 
second, and forty-seventh days. In the deposits were found a small 
number of jointed bacteria — all dead. 
The flasks containing parsnip were opened on the forty-second 
day. <A few needle-like crystals only were observed, imbedded in a 
mass of granular matter. The flasks containing clover infusion were 
examined on the forty-second, forty-seventh, and one hundred and 
sixth days. The deposits were made up of rod-like bacteria, all dead, 
and inorganic matter. 
The next series, consisting of seventeen flasks, was closed June 29th. 
Ten contained hay infusion with cheese added, and seven the same 
infusion without any addition. These were carried to a tempera- 
ture of 145° Cent. (293° F.) and were kept at that point for one and 
one-fourth hours. None of the flasks containing the hay and cheese 
infusions were opened earlier than the forty-second day, and most of 
them were not examined until one hundred days had elapsed. In 
the deposits of all there were found thousands of dead vibriones of the 
necklace type, to the almost entire exclusion of all other organisms. 
On July 23d, thirteen flasks were prepared, five of hay and cheese, 
four of hay to which was added about an equal quantity of stable 
manure, and four of simple hay. These were heated to a tempera- 
ture of 145° Cent. (293° F.)for one hour, and were kept above 100° 
