1878.] 101 {Parker. 
Cent. (212° F.) for two hours. One of the flasks containing hay and 
cheese was opened on the one hundred and eighth day after sealing; 
another was opened on the one hundred and twenty-fourth day; the 
three other flasks containing hay and cheese were broken during the 
process of heating. In those examined, jointed bacteria — all dead — 
were present in limited numbers. The flasks containing hay and 
manure were opened one hundred and thirty days after sealing. 
Only a few cells and oxalate of lime were found amid an ample de- 
posit of inorganic matter. In all these experiments the infusions 
were eximined under the microscope at the time of sealing as al- 
ready mentioned, and a record made of their condition, and a similar 
record when the flasks were opened. 
Following my order of work, I will detail my further experiments 
before giving a comparison of the records. 
In the following experiments the usual course of steeping infusions 
in warm water was changed, boiling water being used in covering the 
substances to be infused; and, instead of suffering the infusions to 
cool, they were boiled for ten minutes in an open vessel and immedi- 
ately filtered and sealed — the entire process occupying not over half 
an hour. This process was adopted because it insures a strong in- 
fusion, and also shuts out the possibility of organism being developed 
in them and multiplying before the final heating. The first of these 
infusions were prepared July 30th, and consisted, four of hay, and 
two of hay and manure.! 
The simple hay infusions were opened on the sixth, fourteenth, and 
one hundred and twenty-second days after sealing. A few dead bac- 
teria were found in the deposits, associated with myriads of inorganic 
particles. The flasks containing hay and manure were opened on 
the seventh and fifteenth days after sealing. In the deposits of both 
these flasks, besides the usual number of bacteria that abound in all 
the deposits from the hay infusions, large numbers of crystalline 
cubes of small size were observed. ‘These had a tendency to ag- 
gregate and form groups which bore a great resemblance to Sarcina. 
Nine flasks were closed Nov. 5th, viz., one of turnip with cheese 
added, one of simple urine, and seven of hay and cheese. These 
were carried to a temperature of 140° Cent. (284°F.) at which 
they remained one hour. The urine was opened and examined on 
the forty-second day. No organisms were present in the deposit. 
1 Stable manure was used because it contains both animal and vegetable matter 
in a state of decomposition. 
