SEPT. 1911.] ITO.—GLEOSPORIUM. OF THE JAP. PERSIMMON. 199 



is produced by abstriction from the end of the conidiophore. 

 The spores are cylindrical or long ellipsoidal, rarely slightly 

 curved, hyaline and one celled or rarely one septated containing 

 one or more oil drops. At both ends they are roundish and 

 not pointed. (Fig. 1.) They measure 18-25 x 4-6 //. 



Fig. 1. Fig. 2. 



I Hi 



W 



Fig. 1. Spores of Gleosporium Kahi. x500 



Fig. 2. Germinating spores of Gleosparium Kahi with appressoriu. x500 



Placed in a drop of water or nutrient solution, the conidia 

 germinate within tw^elve hours throwing out one or two germ- 

 tubes. During the progress, a cross septum is sometimes formed 

 in the middle part of the spore. The appressorium is soon 

 produced at the tip of a germ-tube or hypha and its formation 

 is most pravalent when the spore is sown in a convex drop of 

 water on a slide in a moist chamber, as Hasselbring (10) has 

 already stated in his paper. The appressoria are roundish or 

 polygonal in shape, at first hyaline but later becoming black 

 in colour, provided with a thick wall. They measure 7-9 x 6-8 

 /. (Fig. 2.) 



When the spores from a pure culture of this fungus were 

 sown in a drop of water on the intact persimmon fruit or on 

 a small wounded portion, characteristic symptoms of this disease 

 w^ere observed after five days in both cases ; and in due course 

 of time the spores were produced abundantly. When a ripened 

 stored apple was inoculated with these spores in a similar 

 manner, infection took place readily, producing blackish spots. 



